Kappa Light Chain Secondary Antibody | IGKC secondary antibody
Kappa Light Chain (HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM) (BSA & Azide Free) (MaxLight 750)
Gene Names
IGKC; Km; HCAK1; IGKCD
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
Kappa Light Chain; Monoclonal Secondary Antibody; Kappa Light Chain (HCAK1; Ig Kappa Chain C Region; IGKC; Immunoglobulin KM) (BSA & Azide Free) (MaxLight 750); HCAK1; Immunoglobulin KM; IGKC secondary antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
SPM508
Specificity
Recognizes human kappa light chain. Does not react with rat. Specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for IGKC secondary antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IP: 1-2ug/500ug protein lysate
FC/FACS: 0.5-2ug/million cells
IHC: 0.5-1ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
WB: 0.5-1ug/ml
Applications are based on unconjugated antibody.
FC/FACS: 0.5-2ug/million cells
IHC: 0.5-1ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
WB: 0.5-1ug/ml
Applications are based on unconjugated antibody.
Immunogen
Human B-Lymphoma Cells. Cellular Localization: Cell surface, cytoplasmic and secreted
Conjugate
MaxLight750
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Product Categories/Family for IGKC secondary antibody
References
1. Takahashi H et. al. Pathol Res Prac 189:300-311 (1993). 2. Momose H et. al. Hum Pathol. 23:1115-1119 (1992).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
~22.5kDa
NCBI Official Full Name
Ig kappa chain V-ID region 16
NCBI Official Synonym Full Names
immunoglobulin kappa constant
NCBI Official Symbol
IGKC
NCBI Official Synonym Symbols
Km; HCAK1; IGKCD
UniProt Protein Name
Ig kappa chain V-ID region 16
UniProt Gene Name
IGKV1D-16
UniProt Entry Name
KV109_HUMAN
Uniprot Description
KV109:
Protein type: Immunoglobulin superfamily
Chromosomal Location of Human Ortholog: 2p12
Cellular Component: external side of plasma membrane; extracellular region; extracellular space; plasma membrane
Molecular Function: antigen binding; serine-type endopeptidase activity
Biological Process: B cell receptor signaling pathway; complement activation; complement activation, classical pathway; defense response to bacterium; innate immune response; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of B cell activation; receptor-mediated endocytosis; regulation of immune response; retinal homeostasis
Disease: Immunoglobulin Kappa Light Chain Deficiency
Research Articles on IGKC
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Product Notes
The IGKC igkv1d-16 (Catalog #AAA6236706) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Kappa Light Chain (HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM) (BSA & Azide Free) (MaxLight 750) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Kappa Light Chain can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). IP: 1-2ug/500ug protein lysate FC/FACS: 0.5-2ug/million cells IHC: 0.5-1ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. WB: 0.5-1ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the IGKC igkv1d-16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kappa Light Chain, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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