Testing Data
(Figure 1: Immunohistochemistry on frozen section of human kidney: positive reactivity in renal tubules (epithelium)
)
Mouse anti-Human, Rat K-Cadherin/Cadherin-6 Monoclonal Antibody | anti-CDH6 antibody
Mouse anti K-Cadherin/Cadherin-6
Gene Names
CDH6; CAD6; KCAD; FLJ14176; DKFZp564N1116
Reactivity
Human, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Synonyms
K-Cadherin/Cadherin-6; Monoclonal Antibody; Mouse anti K-Cadherin/Cadherin-6; anti-CDH6 antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2B6
Specificity
2B6 recognizes the extracellular domain of cadherin-6.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-CDH6 antibody
Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
Application Notes
2B6 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen sections when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. 2B6 is also suitable for and immunohistochemistry on praraffin-embedded sections. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:50 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting appliCations.
Source Note
2B6 is a Mouse monoclonal IgG1 antibody obtained by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with affinity purified extracellular domain of Human cadherin-6-GST fusion protein.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Testing Data
(Figure 1: Immunohistochemistry on frozen section of human kidney: positive reactivity in renal tubules (epithelium) )
Testing Data
(Figure 1: Immunohistochemistry on frozen section of human kidney: positive reactivity in renal tubules (epithelium)
)
Testing Data
(Figure 2: Immunohistochemistry on paraffin section of human kidney )
Testing Data
(Figure 2: Immunohistochemistry on paraffin section of human kidney
)
Related Product Information for anti-CDH6 antibody
Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the Catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. Cadherin-6 is expressed in the proximal tubule epithelium of the kidney from which most renal cell carcinomas are derived.
Product Categories/Family for anti-CDH6 antibody
References
Shimazui, T., Oosterwijk, E., Akaza, H., Bringuier, P., Ruijter, E., van Berkel, H., Wakka, J. O., van Bokhoven, A., Debruyne, F. M., and Schalken, J. A. (1998). Expression of cadherin-6 as a novel diagnostic tool to predict prognosis of patients with E-cadherin-absent renal cell carcinoma, Clin Cancer Res 4, 2419-24. Shimazui, T., Oosterwijk-Wakka, J., Akaza, H., Bringuier, P. P., Ruijter, E., Debruyne, F. M., Schalken, J. A., and Oosterwijk, E. (2000). AlteRations in expression of cadherin-6 and E-cadherin during kidney development and in renal cell carcinoma, Eur Urol 38, 331-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88,309 Da
NCBI Official Full Name
cadherin-6 preproprotein
NCBI Official Synonym Full Names
cadherin 6, type 2, K-cadherin (fetal kidney)
NCBI Official Symbol
CDH6
NCBI Official Synonym Symbols
CAD6; KCAD; FLJ14176; DKFZp564N1116
NCBI Protein Information
cadherin-6; OTTHUMP00000115839; OTTHUMP00000220908
UniProt Protein Name
Cadherin-6
Protein Family
UniProt Gene Name
CDH6
UniProt Entry Name
CADH6_HUMAN
NCBI Description
This gene encodes a member of the cadherin superfamily. Cadherins are membrane glycoproteins that mediate homophilic cell-cell adhesion and play critical roles in cell differentiation and morphogenesis. The encoded protein is a type II cadherin and may play a role in kidney development as well as endometrium and placenta formation. Decreased expression of this gene may be associated with tumor growth and metastasis. [provided by RefSeq]
Uniprot Description
CDH6: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Highly expressed in brain, cerebellum, and kidney. Lung, pancreas, and gastric mucosa show a weak expression. Also expressed in certain liver and kidney carcinomas. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5p13.3
Cellular Component: plasma membrane; integral to membrane
Molecular Function: calcium ion binding
Biological Process: intercellular junction assembly and maintenance; cell adhesion; homophilic cell adhesion
Research Articles on CDH6
Similar Products
Product Notes
The CDH6 cdh6 (Catalog #AAA570007) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti K-Cadherin/Cadherin-6 reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's K-Cadherin/Cadherin-6 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB). 2B6 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen sections when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. 2B6 is also suitable for and immunohistochemistry on praraffin-embedded sections. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:50 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting appliCations. Researchers should empirically determine the suitability of the CDH6 cdh6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "K-Cadherin/Cadherin-6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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