Mouse ISL1 Monoclonal Antibody | anti-ISL1 antibody

ISL1 (ISL LIM Homeobox 1, Isl-1) (AP)

Cat. Num. AAA6161626

• Gene Names: ISL1; Isl-1; ISLET1
• Applications: Western Blot
• Purity: Purified
• Synonyms: ISL1; Monoclonal Antibody; ISL1 (ISL LIM Homeobox 1; Isl-1) (AP); ISL LIM Homeobox 1; Isl-1; anti-ISL1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 20000000
• Specificity: Recognizes ISL1.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ISL1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: ISL1 (NP_002193, 63aa-159aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: YCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEP
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ISL1 on HeLa cell. [antibody concentration 10 ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ISL1 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(ISL1 monoclonal antibody (M05), clone 2E7 Western Blot analysis of ISL1 expression in PC-12.)

Related Product Information for anti-ISL1 antibody

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq]

Product Categories/Family for anti-ISL1 antibody

Antibodies; Transcription Factors; Homeobox (HOX)

References

1. ISL-1 is overexpressed in non-Hodgkin lymphoma and promotes lymphoma cell proliferation by forming a p-STAT3/p-c-Jun/ISL-1 complex.Zhang Q, Yang Z, Jia Z, Liu C, Guo C, Lu H, Chen P, Ma K, Wang W, Zhou CMol Cancer. 2014 Jul 29;13(1):181. doi: 10.1186/1476-4598-13-181. 2. A positive feedback regulation of ISL-1 in DLBCL but not in pancreatic beta-cells. Zhang Q, Yang Z, Wang W, Guo T, Jia Z, Ma K, Zhou CBiochem Biophys Res Commun. 2014 Jul 4;449(3):295-300. doi: 10.1016/j.bbrc.2014.05.021. Epub 2014 May 15. 3. Interaction of Wnt/?]-catenin and Notch signaling in the early stage of cardiac differentiation of P19CL6 cells. Li B, Jia Z, Wang T, Wang W, Zhang C, Chen P, Ma K, Zhou C.J Cell Biochem. 2011 Sep 28. doi: 10.1002/jcb.23390. 4. ISL1 Promotes Pancreatic Islet Cell Proliferation. Guo T, Wang W, Zhang H, Liu Y, Chen P, Ma K, Zhou C.PLoS One. 2011;6(8):e22387. Epub 2011 Aug 4.

NCBI and Uniprot Product Information

• NCBI GI #: 115387114
• NCBI GeneID: 3670
• NCBI Accession #: NP_002193
• NCBI GenBank Nucleotide #: NM_002202
• UniProt Accession #: P61371; P20663; P47894
• Molecular Weight: 39,036 Da
• NCBI Official Full Name: insulin gene enhancer protein ISL-1
• NCBI Official Synonym Full Names: ISL LIM homeobox 1
• NCBI Official Symbol: ISL1
• NCBI Official Synonym Symbols: Isl-1; ISLET1
• NCBI Protein Information: insulin gene enhancer protein ISL-1; ISL1 transcription factor, LIM/homeodomain; islet-1
• UniProt Protein Name: Insulin gene enhancer protein ISL-1
• Protein Family: Insulin gene enhancer protein
• UniProt Gene Name: ISL1
• UniProt Synonym Gene Names: Islet-1
• UniProt Entry Name: ISL1_HUMAN

Uniprot Description

ISL1: Binds to one of the cis-acting domain of the insulin gene enhancer.

Protein type: Cell development/differentiation; Nuclear receptor co-regulator; DNA-binding; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q11.1

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; zinc ion binding; bHLH transcription factor binding; estrogen receptor binding; chromatin binding

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of interleukin-12 production; positive regulation of interleukin-1 alpha production; negative regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of estrogen receptor signaling pathway; pancreas development; positive regulation of cell proliferation; ventricular cardiac muscle morphogenesis; neuron fate specification; negative regulation of neuron apoptosis; sensory system development; neural crest cell migration; positive regulation of DNA binding; trigeminal nerve development; pharyngeal system development; axon regeneration; spinal cord motor neuron cell fate specification; positive regulation of insulin secretion; positive regulation of interleukin-6 production; spinal cord motor neuron differentiation; positive regulation of tumor necrosis factor production; positive regulation of interleukin-1 beta production; positive regulation of interferon-gamma production; negative regulation of neuron differentiation; positive regulation of angiogenesis; mesenchymal cell differentiation; visceral motor neuron differentiation; pituitary gland development; negative regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; peripheral nervous system neuron axonogenesis; retinal ganglion cell axon guidance