Mouse IRF4 Monoclonal Antibody | anti-Irf4 antibody

Anti-IRF4 Antibody, Mouse Monoclonal

Cat. Num. AAA8100197

• Gene Names: IRF4; MUM1; LSIRF; NF-EM5
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: IRF4; Monoclonal Antibody; Anti-IRF4 Antibody; Mouse Monoclonal; Irf4 Antibody; Mouse MAb; interferon regulatory factor 4; Anti-LSIRF Antibody; Anti-MUM1 Antibody; Anti-NF-EM5 Antibody; anti-Irf4 antibody

• Host: Mouse
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 01
• Specificity: Human IRF4
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-Irf4 antibody: Flow Cytometry (FC/FACS/FCM)
• Application Notes: FC/FACS/FCM: 1:25-1:100
• Immunogen: A synthetic peptide corresponding to the center region of the Human Irf4
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the Human Irf4. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human IRF4 expression on PHA-stimulated lymphocytes. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human IRF4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-Irf4 antibody

Interferon regulatory factors play an important role in the transcriptional regulation of immunity. In addition to regulating ccr9a expression, Irf4a is essential in T lymphoid-primed progenitors for repressing Pu.1 expression to prevent an alternate fate. The transcription factor IFN regulatory factor (IRF)4 was shown to play a crucial role in the protective CD8(+) T cell response.

NCBI and Uniprot Product Information

• NCBI GI #: 305632828
• NCBI GeneID: 3662
• NCBI Accession #: NP_001182215.1
• NCBI GenBank Nucleotide #: NM_001195286.1
• UniProt Accession #: Q15306; Q5VUI7; Q99660
• Molecular Weight: 51,772 Da
• NCBI Official Full Name: interferon regulatory factor 4 isoform 2
• NCBI Official Synonym Full Names: interferon regulatory factor 4
• NCBI Official Symbol: IRF4
• NCBI Official Synonym Symbols: MUM1; LSIRF; NF-EM5
• NCBI Protein Information: interferon regulatory factor 4; multiple myeloma oncogene 1; lymphocyte-specific interferon regulatory factor
• UniProt Protein Name: Interferon regulatory factor 4
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF4
• UniProt Synonym Gene Names: MUM1; IRF-4; LSIRF
• UniProt Entry Name: IRF4_HUMAN

NCBI Description

The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

IRF4: Transcriptional activator. Binds to the interferon- stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Interacts with SPIB and DEF6. Not induced by interferons. Lymphoid cells. Belongs to the IRF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 6p25-p23

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; sequence-specific DNA binding; protein-lysine N-methyltransferase activity; transcription factor binding; transcription factor activity

Biological Process: T cell activation; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of interleukin-2 biosynthetic process; positive regulation of transcription, DNA-dependent; regulation of T-helper cell differentiation; myeloid dendritic cell differentiation; defense response to protozoan; peptidyl-lysine methylation; positive regulation of interleukin-4 biosynthetic process; positive regulation of interleukin-10 biosynthetic process; positive regulation of interleukin-13 biosynthetic process; negative regulation of toll-like receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA binding

Disease: Skin/hair/eye Pigmentation, Variation In, 8