Mouse anti-Human Interferon gamma, neutralizing Monoclonal Antibody | anti-IFNG antibody

Interferon gamma, neutralizing (IFNg)

Cat. Num. AAA633123

• Gene Names: IFNG; IFG; IFI
• Reactivity: Human
• Applications: ELISA, Neutralization Assay
• Purity: Affinity Purified; Purified by Protein A/S-Sepharose.
• Synonyms: Interferon gamma; neutralizing; Monoclonal Antibody; neutralizing (IFNg); Anti -Interferon gamma; anti-IFNG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9L376
• Specificity: Recognizes human Interferon gamma.
• Purity/Purification: Affinity Purified; Purified by Protein A/S-Sepharose.
• Form/Format: Supplied as lyophilized powder from PBS. Reconstitute with 0.2ml water.

• Applicable Applications for anti-IFNG antibody: ELISA (EL/EIA), Neutralization
• Application Notes: Suitable for use as a neutralizing and binding (ELISA) antibody. This antibody only works in a capture ELISA assay. It does not recognize plastic-bound IFN-gamma, but works very well in capture ELISA when used as the first, plastic bound antibody (and then developed with polyclonal antibody).
• Immunogen: Recombinant human INF-gamma.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-IFNG antibody

Interferon gamma is the only known type II interferon. Under physiological conditions, interferon gamma is induced when T-lymphocytes, previously sensitized to an antigen, are exposed to the same antigen, hence its other name: immune interferon. Interferon gamma has demonstrated significant anti-proliferative effects in in vivo studies; however, its therapeutic effects have been limited. Endogenous IFN-g has been shown to be required for the protection of mice against mycobacteri and leishmaniasis. In the U.S., human IFN-g has been approved for reducing the frequency and severity of infections in patients with chronic granulomatous disease.

Product Categories/Family for anti-IFNG antibody

Antibodies; Abs to Interferon

NCBI and Uniprot Product Information

• NCBI GI #: 184639
• NCBI GeneID: 3458
• UniProt Accession #: P01579; Q53ZV4; B5BU88; Q14609; Q14610; Q14611; Q14612; Q14613; Q14614; Q14615; Q8NHY9; Q96LA2
• Molecular Weight: 19,348 Da
• NCBI Official Full Name: interferon-gamma
• NCBI Official Synonym Full Names: interferon, gamma
• NCBI Official Symbol: IFNG
• NCBI Official Synonym Symbols: IFG; IFI
• NCBI Protein Information: interferon gamma; IFN-gamma; immune interferon; OTTHUMP00000240111
• UniProt Protein Name: Interferon gamma
• Protein Family: Interferon
• UniProt Gene Name: IFNG
• UniProt Entry Name: IFNG_HUMAN

NCBI Description

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.

Uniprot Description

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Cytokine; Secreted; Secreted, signal peptide; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: interferon-gamma receptor binding; cytokine activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of interleukin-12 production; positive regulation of interleukin-23 production; negative regulation of smooth muscle cell proliferation; apoptosis; positive regulation of osteoclast differentiation; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of killing of cells of another organism; negative regulation of transcription from RNA polymerase II promoter; sensory perception of mechanical stimulus; positive regulation of interleukin-1 beta secretion; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; neutrophil chemotaxis; positive regulation of synaptic transmission, cholinergic; adaptive immune response; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; positive regulation of tumor necrosis factor production; defense response to protozoan; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; negative regulation of interleukin-17 production; positive regulation of chemokine biosynthetic process; positive regulation of interleukin-12 biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; cell motility; positive regulation of neuron differentiation; regulation of insulin secretion

Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2; Human Immunodeficiency Virus Type 1, Susceptibility To