Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney TissuePrimary Ab: 10ug/ml Mouse Anti-Human IFNa21 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (MBS2086035))
Mouse anti-Human Interferon Alpha 21 (IFNa21) Monoclonal Antibody | anti-IFNa21 antibody
Monoclonal Antibody to Interferon Alpha 21 (IFNa21)
Gene Names
IFNA21; LeIF F; leIF-F; IFN-alphaI
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G affinity chromatography
Synonyms
Interferon Alpha 21 (IFNa21); Monoclonal Antibody; Monoclonal Antibody to Interferon Alpha 21 (IFNa21); Cytokine; anti-IFNa21 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
D4
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid; supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence
Asp25~Glu189
Sequence Length
189
Applicable Applications for anti-IFNa21 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL
Immunogen
Recombinant IFNa21 (Asp25~Glu189) expressed in E. coli (MBS2011181)
Cross Reactivity
Pig
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney TissuePrimary Ab: 10ug/ml Mouse Anti-Human IFNa21 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (MBS2086035))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney TissuePrimary Ab: 10ug/ml Mouse Anti-Human IFNa21 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (MBS2086035))
Western Blot (WB)
(Sample: Porcine Liver lysatePrimary Ab: 2 ug/ml Mouse Anti-Human IFNa21 AntibodySecondary Ab: 0.2 ug/ml HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (MBS2086032))
Western Blot (WB)
(Sample: Porcine Liver lysatePrimary Ab: 2 ug/ml Mouse Anti-Human IFNa21 AntibodySecondary Ab: 0.2 ug/ml HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (MBS2086032))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interferon alpha-21
NCBI Official Synonym Full Names
interferon alpha 21
NCBI Official Symbol
IFNA21
NCBI Official Synonym Symbols
LeIF F; leIF-F; IFN-alphaI
NCBI Protein Information
interferon alpha-21
UniProt Protein Name
Interferon alpha-21
Protein Family
UniProt Gene Name
IFNA21
UniProt Synonym Gene Names
IFN-alpha-21; LeIF F
NCBI Description
This gene is a member of the alpha interferon gene cluster on the short arm of chromosome 9. Interferons are cytokines produced in response to viral infection that mediate the immune response and interfere with viral replication. The encoded protein is a type I interferon and may play a specific role in the antiviral response to rubella virus. [provided by RefSeq, Sep 2011]
Uniprot Description
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Research Articles on IFNa21
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Product Notes
The IFNa21 ifna21 (Catalog #AAA2111320) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Interferon Alpha 21 (IFNa21) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Interferon Alpha 21 (IFNa21) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL IHC: 5-20ug/mL ICC: 5-20ug/mL. Researchers should empirically determine the suitability of the IFNa21 ifna21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Asp25~Glu1 89. It is sometimes possible for the material contained within the vial of "Interferon Alpha 21 (IFNa21), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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