Testing Data
(MBS600912 staining of mouse gut.)
Rat anti-Mouse Integrin alpha5, beta1 Monoclonal Antibody
Integrin alpha5, beta1 (VLA5, Fibronectin Receptor, Very Late Antigen, CD49)
Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
AscitesAscites
Synonyms
Integrin alpha5; beta1; Monoclonal Antibody; beta1 (VLA5; Fibronectin Receptor; Very Late Antigen; CD49); Anti -Integrin alpha5; anti-Integrin alpha5; beta1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG3
Clone Number
2Q962
Specificity
Recognizes mouse Integrin alpha5, beta1, a fibronectin receptor.
Purity/Purification
Ascites
Ascites
Ascites
Form/Format
Supplied as a liquid.
Applicable Applications for anti-Integrin alpha5, beta1 antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in the Inhibition of cell adhesion to fibronectin, Immunoprecipitation, Flow Cytometry and Western Blot.
Dilution: Inhibition of Cell Adhesion to Fibronectin: 10ul/ml
Note: where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (a4b1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete.
Immunoprecipitation from lysate of 10e6 cell equivalent: 2ul Flow Cytometry: 10ul/ml
Western Blot: Non-denaturing gel
Optimal dilution determined by the researcher.
Dilution: Inhibition of Cell Adhesion to Fibronectin: 10ul/ml
Note: where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (a4b1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete.
Immunoprecipitation from lysate of 10e6 cell equivalent: 2ul Flow Cytometry: 10ul/ml
Western Blot: Non-denaturing gel
Optimal dilution determined by the researcher.
Immunogen
Mouse embryonal carcinoma P19 cells
Hybridoma
Fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(MBS600912 staining of mouse gut.)
Testing Data
(MBS600912 staining of mouse gut.)
Related Product Information for anti-Integrin alpha5, beta1 antibody
Specificity of the antibody was verified by its ability to immunoprecipitate a5b1 heterodimers from 125-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express a5b1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions.
Product Categories/Family for anti-Integrin alpha5, beta1 antibody
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Product Notes
The Integrin alpha5, beta1 (Catalog #AAA600912) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Integrin alpha5, beta1 (VLA5, Fibronectin Receptor, Very Late Antigen, CD49) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin alpha5, beta1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Suitable for use in the Inhibition of cell adhesion to fibronectin, Immunoprecipitation, Flow Cytometry and Western Blot. Dilution: Inhibition of Cell Adhesion to Fibronectin: 10ul/ml Note: where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (a4b1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete. Immunoprecipitation from lysate of 10e6 cell equivalent: 2ul Flow Cytometry: 10ul/ml Western Blot: Non-denaturing gel Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the Integrin alpha5, beta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin alpha5, beta1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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