anti-Human Integrin alpha 5 (CD49e) Monoclonal Antibody | anti-ITGA5 antibody

Mouse anti Integrin alpha 5 (CD49e)

Cat. Num. AAA570237

• Gene Names: ITGA5; FNRA; CD49e; VLA5A
• Reactivity: Human
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: Integrin alpha 5 (CD49e); Monoclonal Antibody; Mouse anti Integrin alpha 5 (CD49e); anti-ITGA5 antibody

• Host: Mouse (Balb/c)
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: NKI-SAM-1
• Specificity: NKI-SAM-1 recognizes the 135-kDa integrin-alpha5 monocyte-specific antigen. The exact epitope is not known. NKI-SAM-1 strongly reacts with monocytes, and weakly with granulocytes, platelets and T-lymphocytes.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-ITGA5 antibody: Flow Cytometry (FC), Immunohistochemistry (IHC) (frozen), Immunoprecipitation (IP)
• Application Notes: NKI-SAM-1 is suitable for flow cytometry (using 0.5-1 ug for 106 cells) and can be used to determine the VLA-5 expression in peripheral blood monocytes and monocyte derived dendritic cells B cells and B-cell precursors. NKI-SAM-1 can be applied in immunohistochemistry, immunocytochemistry (frozen sections) and immunoprecipitation. Optimal antibody dilution should be determined by titRation.
• Source Note: NKI-SAM-1 is a monoclonal antibody obtained by immunization of Balb/c mice with Human monocytes isolated by centrifugal elutriation.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

Testing Data

(Figure 1: Flow cytometric analysis of human monocytes showing CD49e positive population )

Related Product Information for anti-ITGA5 antibody

Integrins are transmembrane glycoproteins that belong to the family of adhesion molecules. They promote interactions between cells and their environment, being both other cells or the extra- cellular matrix. Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated subunits, i.e. an alpha-subunit of 95 kDa that is conserved through the superfamily and a more variable beta subunit of 150-170 kDa. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. The integrin alpha5 chain (160 kDa) (CD49e), undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light (25 kDa) and heavy (135 kDa) chains, which non-covalently associate with the integrin beta1 subunit (CD29) (130 kDa), thus forming the heterodimer alpha5-beta1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor (FNr) that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5 mediates binding of T- and B-cells to fibronectin, an extracellular matrix glycoprotein involved in cell adhesion and migRation in wound healing, embryonic development and malignant transformation. The VLA-5 or FNr binds only fibronectin recognizing the Arg-Gly-Asp (RGD) sequence in the central region of the molecule.

Product Categories/Family for anti-ITGA5 antibody

CD markers; Cell adhesion; Cell adhesion; Immunology

References

Te Velde, A., Klomp, J., Yard, B., De Vries, J. and Figdor, C. (1988). Modulation of phenotypic and functional properties of Human peripheral blood monocytes by IL-4. Journal Immunol 140, 1548-54. Ammon, C., Meyer, S.P., Schwarzfischer, L., Krause, S.W., Andreesen, R. and Kreutz, M. (2000). Comparitive analysis of integrin expression on monocyte-derived macrophages and monocyte-derived dendritic cells. Immunology 100, 364-9. Grassi, F., Dezutter-Dambuyant, C., McIlroy, D., Jacquet, C., Yoneda, K., Imamura, S., Boumsell, L., Schmitt, D., Autran, B., Debre, P., Hosmalin, A. (1998). Monocyte-derived dendritic cells have a phenotype comparable to that of dermal cells and display ultrastructural granules distinct from Birbeck granules. J Leukoc Biol 64, 484-493. Xiao, J., Messinger, Y., Jin, J., Myers, D., Bolen, J. and Uckun, F. (1996). Signal transduction through the b1 integrin Family surface adhesion Molecules VLA-4 and VLA-5 of Human B-cell precursors activates CD19 receptor-associated protein-tyrosine Kinases. J Biol Chem 271, 7659-64. Santoni, G., Birbarelli, P., Hong, L., Gamero, A., Djeu, J. and Piccoli, M. (1995). An a5b1-like integrin receptor mediates the binding of less pathogenic Candida species to fibronectin. J Med Microbiol 43, 360-7. Klingemann, H.G. and Dedhar, S. (1989). Distribution of integrins on Human peripheral blood mononuclear cells. Blood 74, 1348-54.

NCBI and Uniprot Product Information

• NCBI GI #: 56237029
• NCBI GeneID: 3678
• NCBI Accession #: NP_002196.2
• NCBI GenBank Nucleotide #: NM_002205.2
• UniProt Accession #: P08648; Q96HA5
• Molecular Weight: 114,536 Da
• NCBI Official Full Name: integrin alpha-5
• NCBI Official Synonym Full Names: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• NCBI Official Symbol: ITGA5
• NCBI Official Synonym Symbols: FNRA; CD49e; VLA5A
• NCBI Protein Information: integrin alpha-5; VLA-5; integrin alpha-F; CD49 antigen-like family member E; fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; very late activation protein 5, alpha subunit
• UniProt Protein Name: Integrin alpha-5
• Protein Family: Integrin
• UniProt Gene Name: ITGA5
• UniProt Synonym Gene Names: FNRA
• UniProt Entry Name: ITA5_HUMAN

NCBI Description

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGA5: Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with RAB21 and COMP. Interacts with HIV- 1 Tat. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q11-q13

Cellular Component: ruffle; focal adhesion; cell surface; cytoplasm; plasma membrane; synapse; intercellular junction; integrin complex; external side of plasma membrane

Molecular Function: integrin binding; protein binding; vascular endothelial growth factor receptor 2 binding; metal ion binding; platelet-derived growth factor receptor binding; epidermal growth factor receptor binding

Biological Process: integrin-mediated signaling pathway; axon guidance; extracellular matrix organization and biogenesis; viral reproduction; cell-substrate adhesion; positive regulation of vascular endothelial growth factor receptor signaling pathway; memory; heterophilic cell adhesion; heterotypic cell-cell adhesion; leukocyte adhesion; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell adhesion mediated by integrin; cell-substrate junction assembly; angiogenesis; blood coagulation; cell adhesion; leukocyte migration; wound healing, spreading of epidermal cells; positive regulation of cell migration