Mouse anti-Human Insulin Like Growth Factor Binding Protein 1 (IGFBP1) Monoclonal Antibody | anti-IGFBP1 antibody

Monoclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1)

Cat. Num. AAA2090609

• Gene Names: IGFBP1; AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Protein A + Protein G affinity chromatography
• Synonyms: Insulin Like Growth Factor Binding Protein 1 (IGFBP1); Monoclonal Antibody; Monoclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1); IBP1; AFBP; IGF-BP25; PP12; Placental Protein 12; Amniotic Fluid Binding Protein; Alpha-Pregnancy-Associated Endometrial Globulin; Growth Hormone Independent-Binding Protein; anti-IGFBP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b Kappa
• Clone Number: C3
• Specificity: The antibody is a mouse monoclonal antibody raised against IGFBP1. It has been selected for its ability to recognize IGFBP1 in immunohistochemical staining and western blotting.
• Purity/Purification: Protein A + Protein G affinity chromatography
• Form/Format: Liquid
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 259

• Applicable Applications for anti-IGFBP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: Western Blot: 0.5-5ug/mL; Immunohistochemistry: 5-30ug/mL; Immunocytochemistry: 5-30ug/mL
• Immunogen: Recombinant Insulin Like Growth Factor Binding Protein 1 (IGFBP1)
• Cross Reactivity: Human
• Buffer Formulation: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant IGFBP1, Human.)

NCBI and Uniprot Product Information

• NCBI GI #: 4504615
• NCBI GeneID: 3484
• NCBI Accession #: NP_000587.1
• NCBI GenBank Nucleotide #: NM_000596.3
• UniProt Accession #: P08833; Q8IYP5; A4D2F4; D3DVL9
• Molecular Weight: 27,904 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 1
• NCBI Official Synonym Full Names: insulin like growth factor binding protein 1
• NCBI Official Symbol: IGFBP1
• NCBI Official Synonym Symbols: AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
• NCBI Protein Information: insulin-like growth factor-binding protein 1
• UniProt Protein Name: Insulin-like growth factor-binding protein 1
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP1
• UniProt Synonym Gene Names: IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; PP12

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP N-terminal domain and a thyroglobulin type-I domain. The encoded protein, mainly expressed in the liver, circulates in the plasma and binds both insulin-like growth factors (IGFs) I and II, prolonging their half-lives and altering their interaction with cell surface receptors. This protein is important in cell migration and metabolism. Low levels of this protein may be associated with impaired glucose tolerance, vascular disease and hypertension in human patients. [provided by RefSeq, Aug 2017]

Uniprot Description

IGFBP1: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi apparatus

Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor II binding; protein binding; receptor binding

Biological Process: aging; cellular protein metabolic process; insulin receptor signaling pathway; negative regulation of canonical Wnt signaling pathway; PERK-mediated unfolded protein response; positive regulation of cell growth; post-translational protein modification; regulation of insulin-like growth factor receptor signaling pathway; signal transduction; tissue regeneration