Mouse Influenza A H1N1 Hemagglutinin/HA Monoclonal Antibody | anti-HA antibody

Influenza A H1N1 (Swine Flu 2009) Hemagglutinin/HA Antibody, Mouse MAb

Cat. Num. AAA8104732

• Reactivity: H1N1
• Applications: Western Blot, ELISA
• Purity: Protein A
• Synonyms: Influenza A H1N1 Hemagglutinin/HA; Monoclonal Antibody; Influenza A H1N1 (Swine Flu 2009) Hemagglutinin/HA Antibody; Mouse MAb; Harvey rat sarcoma viral oncogene homolog; anti-HA antibody

• Host: Mouse
• Reactivity: H1N1
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 9G1G8
• Specificity: H1N1 (A/California/04/2009) HA; H1N1 (A/California/07/2009) HA.; Has cross-reactivity in ELISA with H1N2 (A/swine/Guangxi/13/2006) HA; H1N3 (A/duck/NZL/160/1976) HA; H5N1 (A/Anhui/1/2005) HA; H5N1 (A/Viet nam/1194/2004) HA; H5N1 (A/Indonesia/5/2005) HA; H5N1 (A/turkey/Turkey/1/2005) HA; H5N1 (A/bar-headed goose/Qinghai/14/2008) HA.; No cross-reactivity in ELISA with H1N1 (A/Brisbane/59/2007) HA; H1N1 (A/BrevigMission/1/1918) HA; H1N1 (A/Solomon Islands/3/2006) HA; H1N1 (A/Ohio/UR06-0091/2007) HA; H1N1 (A/New Caledonia/20/1999) HA; H1N1 (A/Puerto Rico/8/1934) HA; H1N1 (A/WSN/1933) HA; H3N2 (A/Brisbane/10/2007) HA; Influenza B (B/Florida/4/2006) HA; Has cross-reactivity in ELISA with H1N2 (A/swine/Guangxi/13/2006) HA; H1N3 (A/duck/NZL/160/1976) HA; H5N1 (A/Anhui/1/2005) HA; H5N1 (A/Viet nam/1194/2004) HA; H5N1 (A/Indonesia/5/2005) HA; H5N1 (A/turkey/Turkey/1/2005) HA; H5N1 (A/bar-headed goose/Qinghai/14/2008) HA
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-HA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: This antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect H1N1 HA in WB.; ELISA: 1:1000-1:2000; FC/FACS/FCM: 1:25-1:100; Antibody's applications haven't been validated with corresponding virus positive samples.
• Immunogen: Recombinant H1N1 HA protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza A virus H1N1 hemagglutinin (HA) extracellular domain. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Testing Data

(High Five cells were infected by Bac-HA, cells were collected at 48 hours postinfection, and the cell surface expression of HA were measured by flow cytometry. 106 Cells were stained with 1 ug Purified Mouse Anti-H1N1-HA (11055-MM08) antibody for 20 min on ice. Cells were washed twice and incubated with 1 ug of a FITC Goat Anti-Mouse Ig secondary antibody for 20 min on ice. Cells were washed twice and analyzed by flow cytometry.)

Related Product Information for anti-HA antibody

The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

References

White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
Donald J. Benton, Influenza hemagglutinin membrane anchor, PNAS, 2018