Mouse Infectious Peritonitis, Feline Monoclonal Antibody
Infectious Peritonitis, Feline (FIPV)
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Synonyms
Infectious Peritonitis; Feline; Monoclonal Antibody; Feline (FIPV); Anti -Infectious Peritonitis; anti-Infectious Peritonitis; Feline antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3H2460
Specificity
Recognizes feline infectious peritonitis virus (FIPV) type 1 and 2. Specific for the nucleocapsid. Shown to react with canine coronavirus (CCV), porcine coronavirus transmissable gastroenteritis virus (TGEV) and ferret coronavirus. Some reactivity has been observed against bovine coronavirus (BCV). Exhibits negative reactivity against feline leukemia virus, feline immunodeficiency virus, feline calcivirus, feline Herpes virus, canine adenovirus (type 2), canine distemper virus, canine parvovirus and canine parainfluenza virus.
Purity/Purification
Purified by Protein A affinity chromatography from tissue culture supernatant.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Infectious Peritonitis, Feline antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Immunohistochemistry (Frozen/paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Sodium citrate buffer, pH 6.0 is recommended.
Western Blot (Reducing conditions): Detects a band of 50-56kD that represents the nucleocapsid. Also detects specific bands at 56 and 46kD in reducing gels with canine coronavirus (CCV) as the antigen.
Optimal dilutions to be determined by the researcher.
Western Blot (Reducing conditions): Detects a band of 50-56kD that represents the nucleocapsid. Also detects specific bands at 56 and 46kD in reducing gels with canine coronavirus (CCV) as the antigen.
Optimal dilutions to be determined by the researcher.
Grade
Affinity Purified
Immunogen
Coronavirus cocktail.
Recommended Pairs
Capture: MBS602186
Detection: Please Inquire
Detection: Please Inquire
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Product Categories/Family for anti-Infectious Peritonitis, Feline antibody
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Product Notes
The Infectious Peritonitis, Feline (Catalog #AAA602186) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Infectious Peritonitis, Feline can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Immunohistochemistry (Frozen/paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Sodium citrate buffer, pH 6.0 is recommended. Western Blot (Reducing conditions): Detects a band of 50-56kD that represents the nucleocapsid. Also detects specific bands at 56 and 46kD in reducing gels with canine coronavirus (CCV) as the antigen. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Infectious Peritonitis, Feline for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Infectious Peritonitis, Feline, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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