Mouse INDOLEAMINE 2,3-DIOXYGENASE Monoclonal Antibody | anti-IDO1 antibody

MOUSE ANTI HUMAN INDOLEAMINE 2,3-DIOXYGENASE

Cat. Num. AAA213447

• Gene Names: IDO1; IDO; INDO; IDO-1
• Applications: Immunohistochemistry, Western Blot
• Synonyms: INDOLEAMINE 2; 3-DIOXYGENASE; Monoclonal Antibody; MOUSE ANTI HUMAN INDOLEAMINE 2; anti-IDO1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3
• Clone Number: 10.1
• Specificity: This item recognizes Indoleamine-pyrrole 2,3 - dioxygenase (IDO), an enzyme responsible for the catabolism of tryptophan to N-formylkynurenine and kynurenine. With the exception of tryptophan oxidase in hepatocytes, IDO is the only enzyme that degrades the amino acid tryptophan to kynurenine. Chlamydial pathogens require tryptophan for growth. IDO can lower available tryptophan in target cells leading to conversion of the pathogen to persistant forms, with significance in the progression to chronic infection with its associated pathological effects.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 403

• Applicable Applications for anti-IDO1 antibody: Immunohistology Frozen, Western Blot (WB)
• Application Notes: Western Blot: This item recognizes a 45kDa band in IFN-gamma treated human cell lines. This antibody recognizes a slightly lower molecular weight band in Mouse IDO compared to its human counterpart.
• Perservative Stabilisers: 0.1% Sodium Azide (NaN3)
• Immunogen: Peptide corresponding to amino acids 78-184 of human IDO fused to GST.; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-IDO1 antibody

MBS213447 recognizes Indoleamine-pyrrole 2,3 - dioxygenase (IDO), an enzyme responsible for the catabolism of tryptophan to N-formylkynurenine and kynurenine. With the exception of tryptophan oxidase in hepatocytes, IDO is the only enzyme that degrades the amino acid tryptophan to kynurenine. Chlamydial pathogens require tryptophan for growth. IDO can lower available tryptophan in target cells leading to conversion of the pathogen to persistant forms, with significance in the progression to chronic infection with its associated pathological effects.

NCBI and Uniprot Product Information

• NCBI GI #: 4504577
• NCBI GeneID: 3620
• NCBI Accession #: NP_002155.1
• NCBI GenBank Nucleotide #: NM_002164.5
• UniProt Accession #: P14902; Q540B4
• Molecular Weight: 45,326 Da
• NCBI Official Full Name: indoleamine 2,3-dioxygenase 1
• NCBI Official Synonym Full Names: indoleamine 2,3-dioxygenase 1
• NCBI Official Symbol: IDO1
• NCBI Official Synonym Symbols: IDO; INDO; IDO-1
• NCBI Protein Information: indoleamine 2,3-dioxygenase 1; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine-pyrrole 2,3-dioxygenase
• UniProt Protein Name: Indoleamine 2,3-dioxygenase 1
• Protein Family: Indoleamine 2,3-dioxygenase
• UniProt Gene Name: IDO1
• UniProt Synonym Gene Names: IDO; INDO; IDO-1
• UniProt Entry Name: I23O1_HUMAN

NCBI Description

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]

Uniprot Description

INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family.

Protein type: Oxidoreductase; Cell cycle regulation; EC 1.13.11.52; Amino Acid Metabolism - tryptophan

Chromosomal Location of Human Ortholog: 8p12-p11

Cellular Component: stereocilium bundle; smooth muscle contractile fiber; cytosol

Molecular Function: electron carrier activity; metal ion binding; heme binding; indoleamine 2,3-dioxygenase activity; tryptophan 2,3-dioxygenase activity

Biological Process: regulation of activated T cell proliferation; positive regulation of T-helper 2 type immune response; negative regulation of interleukin-10 production; positive regulation of chronic inflammatory response; tryptophan catabolic process to kynurenine; positive regulation of interleukin-12 production; response to lipopolysaccharide; female pregnancy; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; multicellular organismal response to stress; tryptophan catabolic process; positive regulation of T cell tolerance induction