Mouse anti-Human INCENP Monoclonal Antibody | anti-INCENP antibody

Anti-INCENP Mouse mAb

Cat. Num. AAA476325

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Synonyms: INCENP; Monoclonal Antibody; Anti-INCENP Mouse mAb; anti-INCENP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3D2
• Form/Format: Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
• Sequence Length: 914

• Applicable Applications for anti-INCENP antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Immunogen: Purified recombinant fragment of human INCENP (AA: 369-583) expressed in E Coli.

Western Blot (WB)

(Western blot analysis using INCENP mAb against human INCENP recombinant protein. (Expected MW is 50.2 kDa))

Western Blot (WB)

(Western blot analysis using INCENP mAb against HEK293 (1) and INCENP (AA)

Immunofluorescence (IF)

(Immunofluorescence analysis of HepG2 cells using INCENP mouse mAb (green). Blue)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells using INCENP mouse mAb (green) and negative control (red).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using INCENP mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using INCENP mouse mAb with DAB staining.)

Related Product Information for anti-INCENP antibody

Entrez Summary: In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger, ' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis

NCBI and Uniprot Product Information

• NCBI GI #: 102467242
• NCBI GeneID: 3619
• NCBI Accession #: NP_064623.2
• NCBI GenBank Nucleotide #: NM_020238.2
• UniProt Accession #: Q9NQS7; Q5Y192; A8MQD2
• Molecular Weight: 105.4
• NCBI Official Full Name: inner centromere protein isoform 2
• NCBI Official Synonym Full Names: inner centromere protein
• NCBI Official Symbol: INCENP
• NCBI Protein Information: inner centromere protein
• UniProt Protein Name: Inner centromere protein
• Protein Family: Inner centromere protein
• UniProt Gene Name: INCENP

NCBI Description

In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM, Mar 2008]

Uniprot Description

INCENP: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1. Homodimer or heterodimer. Interacts with H2AFZ. Interacts with CBX3. Interacts with tubulin beta chain. Interacts with AURKB and AURKC. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC. Interacts with EVI5. Interacts with CDCA8 and BIRC5; interaction is direct. Interacts with CBX5; POGZ and INCENP compete for interaction with CBX5. Interacts with POGZ. Interacts with JTB. Belongs to the INCENP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; EC 2.7.11.1

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: centric heterochromatin; chromosome, pericentric region; cytosol; kinetochore; nucleoplasm; protein complex; spindle

Molecular Function: protein binding

Biological Process: chromosome segregation; cytokinesis; protein sumoylation; sister chromatid cohesion