Mouse anti-Human Immunoglobulin-Like Transcript 2 Monoclonal Antibody | anti-ILT-2 antibody

Immunoglobulin-Like Transcript 2 (LT2, Leukocyte Immunoglobulin-like Receptor Subfamily B Member 1, LIR-1, Leukocyte Immunoglobulin-like Receptor 1, CD85 Antigen-like Family Member J, ILT-2, Monocyte/Macrophage Immunoglobulin-like Receptor 7, MIR-7, CD85j

Cat. Num. AAA6250971

• Gene Names: LILRB1; ILT2; LIR1; MIR7; CD85J; ILT-2; LIR-1; MIR-7
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by protein G affinity chromatography from tissue culture supernatant.
• Synonyms: Immunoglobulin-Like Transcript 2; Monoclonal Antibody; Immunoglobulin-Like Transcript 2 (LT2; Leukocyte Immunoglobulin-like Receptor Subfamily B Member 1; LIR-1; Leukocyte Immunoglobulin-like Receptor 1; CD85 Antigen-like Family Member J; ILT-2; Monocyte/Macrophage Immunoglobulin-like Receptor 7; MIR-7; CD85j; anti-ILT-2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4F9
• Specificity: Recognizes human Immunoglobulin-like Transcript 2 expressed predominately on B-cells and monocytes, and at lower levels on dendritic cells, T-cells and natural killer (NK) cells.
• Purity/Purification: Purified by protein G affinity chromatography from tissue culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-ILT-2 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Immunogen: Monocyte derived dendritic cells.
• Conjugate: HRP
• Preparation and Storage: Store at -20 degree C.

Product Categories/Family for anti-ILT-2 antibody

Antibodies; Receptors

References

1. Colonna, M. et al. (1999) A novel family of Ig-like receptors for HLA class I molecules that modulate function of lymphoid and myeloid cells. J. Leukoc. Biol. 66: 375-381. 2. Borges, L. et al. (1997) A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules. J. Immunol. 159: 5192-5196.

NCBI and Uniprot Product Information

• NCBI GI #: 612149795
• NCBI GeneID: 10859
• NCBI Accession #: NP_001075108.2
• NCBI GenBank Nucleotide #: NM_001081639.3
• UniProt Accession #: Q8NHL6; O75024; O75025; Q8NHJ9; Q8NHK0; A2IXV4; A8MXT0
• Molecular Weight: 70,819 Da
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily B member 1 isoform 4
• NCBI Official Synonym Full Names: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
• NCBI Official Symbol: LILRB1
• NCBI Official Synonym Symbols: ILT2; LIR1; MIR7; CD85J; ILT-2; LIR-1; MIR-7
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily B member 1; Ig-like transcript 2; CD85 antigen-like family member J; monocyte/macrophage immunoglobulin-like receptor 7; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 1
• UniProt Gene Name: LILRB1
• UniProt Synonym Gene Names: ILT2; LIR1; MIR7; LIR-1; Leukocyte immunoglobulin-like receptor 1; ILT-2; MIR-7
• UniProt Entry Name: LIRB1_HUMAN

NCBI Description

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LILRB1: Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: cytoplasm; extracellular region; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein homodimerization activity; MHC class I receptor activity; MHC class I protein binding; HLA-A specific inhibitory MHC class I receptor activity; SH2 domain binding; HLA-B specific inhibitory MHC class I receptor activity; protein phosphatase 1 binding

Biological Process: immune response-inhibiting cell surface receptor signaling pathway; regulation of immune response; negative regulation of calcium ion transport; negative regulation of mononuclear cell proliferation; positive regulation of apoptosis; negative regulation of endocytosis; response to virus; negative regulation of natural killer cell mediated cytotoxicity; signal transduction; negative regulation of cytokine secretion during immune response; negative regulation of interferon-gamma biosynthetic process; negative regulation of cell cycle; negative regulation of serotonin secretion; negative regulation of alpha-beta T cell activation; negative regulation of T cell proliferation; T cell proliferation during immune response; receptor internalization; negative regulation of interferon-gamma production; negative regulation of T cell mediated cytotoxicity; interferon-gamma production; negative regulation of tumor necrosis factor biosynthetic process; Fc receptor mediated inhibitory signaling pathway; positive regulation of cytolysis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of defense response to virus by host; defense response to virus