Mouse ILK Monoclonal Antibody | anti-ILK antibody

Anti-ILK Mouse mAb

Cat. Num. AAA476352

• Gene Names: ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• Reactivity: Human, Mouse, Monkey
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: ILK; Monoclonal Antibody; Anti-ILK Mouse mAb; P59; ILK-2; anti-ILK antibody

• Host: Mouse
• Reactivity: Human, Mouse, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3A9
• Form/Format: Ascitic fluid containing 0.03% sodium azide.
• Sequence Length: 452

• Applicable Applications for anti-ILK antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Immunogen: Purified recombinant fragment of human ILK (AA: 97-244) expressed in E Coli.

Western Blot (WB)

(Western blot analysis using ILK mAb against human ILK recombinant protein. (Expected MW is 42.7 kDa))

Western Blot (WB)

(Western blot analysis using ILK mouse mAb against Jurkat (1), NIH3T3 (2), HeLa (3), PC-12 (4), C6 (5), COS7 (6), Raji (7), K562 (8) and MCF-7 (9) cell lysate.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells using ILK mouse mAb (green) and negative control (red).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ILK mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ILK mouse mAb with DAB staining.)

Related Product Information for anti-ILK antibody

Entrez Summary: Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 47115283
• NCBI GeneID: 3611
• Molecular Weight: 51.4
• NCBI Official Full Name: ILK, partial
• NCBI Official Synonym Full Names: integrin linked kinase
• NCBI Official Symbol: ILK
• NCBI Official Synonym Symbols: P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• NCBI Protein Information: integrin-linked protein kinase
• UniProt Protein Name: Integrin-linked protein kinase
• Protein Family: Integrin-linked protein kinase
• UniProt Gene Name: ILK
• UniProt Synonym Gene Names: ILK1; ILK2

NCBI Description

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

ILK: a tyrosine kinase-like kinase of the MLK family. Couples integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. Functions as a scaffold bridging the extra-cellular matrix and growth factor receptors to the actin cytoskeleton through interactions with the ILK-PINCH complex. This complex, which includes integrin, PINCH (which links ILK to receptor tyrosine kinases via Nck2), PARVA and affixin, is considered to be one of the convergence points of integrin- and growth factor-signaling pathways. May be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Stimulated downstream of PI3 kinase. Increased expression is correlated with progression of several tumor types, including breast, prostate, and colon carcinomas. Overexpression drives anchorage-independent growth and faster cell cycle.

Protein type: EC 2.7.11.1; ILK subfamily; Kinase, protein; MLK family; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: cell junction; cytoplasm; cytosol; focal adhesion; membrane; nucleoplasm; stress fiber

Molecular Function: protein binding; protein kinase binding; protein serine/threonine kinase activity; signal transducer activity

Biological Process: cell-matrix adhesion; integrin-mediated signaling pathway; MAPKKK cascade; multicellular organismal development; positive regulation of phosphorylation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation