Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RA antibody SE2 (MBS488768) Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of SE2 (MBS488768) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488768, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Mouse anti-Human IL-8RA Monoclonal Antibody | anti-IL-8RA antibody
Anti-IL-8RA [SE2]
Gene Names
CXCR1; C-C; CD128; CD181; CKR-1; IL8R1; IL8RA; CMKAR1; IL8RBA; CDw128a; C-C-CKR-1
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Purified antibody. Protein A affinity purified
Synonyms
IL-8RA; Monoclonal Antibody; Anti-IL-8RA [SE2]; CXCR-1; C-X-C chemokine receptor type 1; CXC-R1; CDw128a; High affinity interleukin-8 receptor A; IL-8R A; IL-8 receptor type 1; CD181; interleukin 8; anti-IL-8RA antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2b, kappa
Clone Number
SE2
Specificity
SE2 antibody is specific for IL-8RA and does not corss-react with IL-8RB. IL-8RA is a receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IL-8RA antibody
Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes
This antibody is recommended for the detection and analysis of IL-8RA expression. One group used it to identify IL-8RA on the surface of human granulocytes via FACS (Zahn et al., 1997). Importantly, SE2 antibody was specific to IL-8RA-expressing cells only. Subsequent flow cytometric analysis with this antibody led to the characterization of IL-8RA density on human leukocytes (Zahn et al., 1997).
PMID
16144840
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RA antibody SE2 (MBS488768) Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of SE2 (MBS488768) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488768, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RA antibody SE2 (MBS488768) Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of SE2 (MBS488768) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488768, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Western Blot (WB)
(Western Blot using anti-IL-8RA antibody SE2 (MBS488768) Human lymph node tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SE2 (MBS488768) at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-IL-8RA antibody SE2 (MBS488768) Human lymph node tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SE2 (MBS488768) at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RA antibody SE2 (MBS488768) Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of SE2 (MBS488768) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488768, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RA antibody SE2 (MBS488768) Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of SE2 (MBS488768) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488768, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-IL-8RA antibody
Recombinant monoclonal antibody to IL-8RA. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma SE2.
This antibody was raised by immunizing BALB/c mice with a BSA-conjugated peptide comprising of the N-terminal part of IL-8RA (amino acids 1-30).
This antibody was raised by immunizing BALB/c mice with a BSA-conjugated peptide comprising of the N-terminal part of IL-8RA (amino acids 1-30).
Product Categories/Family for anti-IL-8RA antibody
References
Zahn et al. Chemoattractant receptors for interleukin-8 and C5a: expression on peripheral blood leukocytes and differential regulation on HL-60 and AML-193 cells by vitamin D3 and all-trans retinoic acid. Eur J Immunol. 1997 Apr;27(4):935-40.
Note on publication: This article describes the generation and characterization of SE2 antibody.
Note on publication: This article describes the generation and characterization of SE2 antibody.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
39,791 Da
NCBI Official Full Name
CXCR1
NCBI Official Synonym Full Names
chemokine (C-X-C motif) receptor 1
NCBI Official Symbol
CXCR1
NCBI Official Synonym Symbols
C-C; CD128; CD181; CKR-1; IL8R1; IL8RA; CMKAR1; IL8RBA; CDw128a; C-C-CKR-1
NCBI Protein Information
C-X-C chemokine receptor type 1; CXC-R1; CXCR-1; IL-8R A; IL-8 receptor type 1; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A; high affinity interleukin-8 receptor A
UniProt Protein Name
C-X-C chemokine receptor type 1
UniProt Gene Name
CXCR1
UniProt Synonym Gene Names
CMKAR1; IL8RA; CXC-R1; CXCR-1; IL-8R A
UniProt Entry Name
CXCR1_HUMAN
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Product Notes
The IL-8RA cxcr1 (Catalog #AAA488769) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL-8RA [SE2] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-8RA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC). This antibody is recommended for the detection and analysis of IL-8RA expression. One group used it to identify IL-8RA on the surface of human granulocytes via FACS (Zahn et al., 1997). Importantly, SE2 antibody was specific to IL-8RA-expressing cells only. Subsequent flow cytometric analysis with this antibody led to the characterization of IL-8RA density on human leukocytes (Zahn et al., 1997). Researchers should empirically determine the suitability of the IL-8RA cxcr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-8RA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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