Rat anti-Mouse IL-5 Monoclonal Antibody | anti-IL5 antibody

Rat Anti-Mouse IL-5-LE/AF

Cat. Num. AAA670071

• Gene Names: IL5; TRF; IL-5
• Reactivity: Mouse
• Applications: ELISA, Flow Cytometry, Immunofluorescence, Western Blot
• Synonyms: IL-5; Monoclonal Antibody; Rat Anti-Mouse IL-5-LE/AF; Rat Anti-Mouse IL-5 - Low Endotoxin/Azide-Free; Rat Anti-Mouse IL-5; anti-IL5 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG1 kappa
• Clone Number: TRFK5
• Specificity: Mouse and Human Interleukin-5 (IL-5)1-4 (This is a neutralizing antibody.)
• Form/Format: Low Endotoxin, Azide - Free (LE/AF)
• Sequence Length: 134

• Applicable Applications for anti-IL5 antibody: ELISA; Flow Cytometry; Immunofluorescence; Western Blot
• Application Notes: Research Application(s): ELISA Capture: TRFK5 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying mouse or human IL-5 protein levels.5-7 Purified TRFK5 antibody should be paired with biotinylated TRFK4 antibody as the detection antibody, with purified recombinant mouse IL-5 as the standard.; Immunofluorescence/Flow Cytometry: TRFK5 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.8 Recombinant mouse IL-5 or Rat IgG1 should be used as a specificity control.; Western Blotting: The purified TRFK5 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-5 under reducing conditions.; Neutralization: The LE/AF form of TRFK5 can be used for neutralizing mouse IL-5 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
• Immunogen: Semi-purified mouse T cell clone supernatant.
• Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Schumacher, J.H., A. O'garra. B. Schrader, A. Van Kimmenade, M.W. Bond, T.R. Mosmann, and R.L. Coffman. 1988. J. Immunol. 141:1576.
2. Swain, S., A. Welberg, M. English, and G. Houston. 1990. J. Immunol. 145:3796.
3. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5.
4. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. J. Immunol. Meth. 166:201.
5. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.
6. Mo, X.Y., S. R. Sarawar, and P. Doherty. 1995. J. Virol. 69:1288.
7. Sarawar, S. M. Sangster, R. Coffman, and P. Doherty. 1994. J. Immunol. 153:1246.
8. Assenmacher, M., J. Schmitz, and A. Radbruch. 1994. Eur. J. Immunol. 24:1097.

NCBI and Uniprot Product Information

• NCBI GI #: 27806873
• NCBI GeneID: 280825
• NCBI Accession #: NP_776347.1
• NCBI GenBank Nucleotide #: NM_173922.1
• UniProt Accession #: P52173; A9QWQ8
• Molecular Weight: 15,229 Da
• NCBI Official Full Name: interleukin-5
• NCBI Official Symbol: IL5
• NCBI Official Synonym Symbols: TRF; IL-5
• NCBI Protein Information: interleukin-5; T-cell replacing factor; eosinophil differentiation factor; colony-stimulating factor, eosinophil
• UniProt Protein Name: Interleukin-5
• Protein Family: Interleukin
• UniProt Gene Name: IL5
• UniProt Entry Name: IL5_BOVIN

Uniprot Description

Function: Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells

Subunit structure: Homodimer; disulfide-linked

Subcellular location: Secreted

Sequence similarities: Belongs to the IL-5 family.