Mouse anti-Human IL-2 receptor, soluble Monoclonal Antibody | anti-IL-2 receptor antibody

Mouse Anti-Human IL-2 receptor, soluble

Cat. Num. AAA690327

• Gene Names: IL2RA; CD25; IL2R; TCGFR; IDDM10
• Reactivity: Human
• Purity: Protein G chromatography
• Synonyms: IL-2 receptor; soluble; Monoclonal Antibody; Mouse Anti-Human IL-2 receptor; Anti-human sIL-2R alpha; anti-IL-2 receptor antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Purity/Purification: Protein G chromatography
• Form/Format: Lyophilized
• Sequence Length: 272

• Application Notes: Western Blot: To detect human sIL-2Ralpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human sIL-2Ralpha is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
• Antibody Generation: Produced in mice using highly pure (>98%) recombinant human soluble IL-2 Receptor alpha as the immunizing antigen. This IgG2aK antibody was purified from cell culture by Protein G affinity chromatography.
• Buffer: PBS, pH 7.2
• Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hsIL-2Ralpha (10.0 ug/ml), a concentration of 1.0-1.5 ug/ml of this antibody is required, in the presence of 0.5 ng/ml recombinant hIL-2.

Related Product Information for anti-IL-2 receptor antibody

Human IL-2 exerts its biological effects via signaling through its receptor system, IL-2R. IL-2 and its receptor (IL-2R) are required for T-cell proliferation and other fundamental functions which are crucial of the immune response. IL-2R consists of 3 noncovalently linked type I transmembrane proteins which are the alpha (p55), beta (p75), and gamma (p65) chains. The IL-2R alpha chain contains an extracellular domain of 219 amino acids, a transmembrane domain of 19 amino acids, and an intracellular domain of 13 amino acids. The secreted extracellular domain of IL-2R alpha (s-IL-2R-a), also called TAC-antigen, is expressed on leukemia cells, lymphoma cells, approximately 10% NK cells, as well as recently activated T and B cells. Recombinant human s-IL-2R-a is a 24.8 kDa protein containing 219 amino acid residues consisting of only the extracellular domain of IL-2R alpha. Due to glycosylation, IL-2R alpha has an approximate molecular weight of 31 kDa based on SDS-PAGE gel and Mass Spectrometry.

NCBI and Uniprot Product Information

• NCBI GI #: 4557667
• NCBI GeneID: 3559
• NCBI Accession #: NP_000408.1
• NCBI GenBank Nucleotide #: NM_000417.2
• UniProt Accession #: P01589; Q5W007
• Molecular Weight: 30,819 Da
• NCBI Official Full Name: interleukin-2 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 2 receptor, alpha
• NCBI Official Symbol: IL2RA
• NCBI Official Synonym Symbols: CD25; IL2R; TCGFR; IDDM10
• NCBI Protein Information: interleukin-2 receptor subunit alpha; p55; IL2-RA; IL-2-RA; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha; insulin-dependent diabetes mellitus 10
• UniProt Protein Name: Interleukin-2 receptor subunit alpha
• UniProt Gene Name: IL2RA
• UniProt Synonym Gene Names: IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
• UniProt Entry Name: IL2RA_HUMAN

NCBI Description

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

Uniprot Description

Function: Receptor for interleukin-2.

Subunit structure: Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Subcellular location: Membrane; Single-pass type I membrane protein.

Involvement in disease: Diabetes mellitus, insulin-dependent, 10 (IDDM10) [MIM:601942]: A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical features are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.13

Sequence similarities: Contains 2 Sushi (CCP/SCR) domains.