Rat anti-Mouse IL-12 Monoclonal Antibody | anti-IL-12 antibody

Rat Anti-Mouse IL-12

Gene Names

Il12a; p35; Ll12a; Il-12a; IL-12p35

Reactivity

Mouse

Purity

Protein A chromatography

Synonyms

IL-12; Monoclonal Antibody; Rat Anti-Mouse IL-12; Monoclonal antibody anti-murine IL-12; anti-IL-12 antibody

Ordering
Cat. Num. AAA690325 When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures. For Research Use Only!

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Isotype

IgG2

Purity/Purification

Protein A chromatography

Form/Format

Lyophilized

Sequence Length

236

Application Notes

ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 3-4 ug/ml of this antibody will detect at least 0.1 ng/well of recombinant murine IL-12 when used with PeproTech's biotinylated antigen affinity purified anti-murine IL-12 as the detection antibody at a concentration of at least 0.5ug/ml.

Antibody Generation

Monoclonal antibodies were produced in rats using recombinant murine IL-12 as the immunizing antigen. The IgG antibody was purified from ascites fluid followed by Protein G affinity chromatography.

Neutralization

To yield one-half maximal inhibition [ND50] of the biological activity of mIL-12 (2.0 ng/ml), a concentration of 0.16 - 0.24 ug/ml of this antibody is required.

Related Product Information for anti-IL-12 antibody

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

NCBI and Uniprot Product Information

NCBI GI #

226874945

NCBI GeneID

16159

NCBI Accession #

NP_001152896.1

NCBI GenBank Nucleotide #

NM_001159424.1

UniProt Accession #

P43431

Molecular Weight

24,179 Da

NCBI Official Full Name

interleukin-12 subunit alpha isoform 1

NCBI Official Synonym Full Names

interleukin 12a

NCBI Official Symbol

Il12a

NCBI Official Synonym Symbols

p35; Ll12a; Il-12a; IL-12p35

NCBI Protein Information

interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit

UniProt Protein Name

Interleukin-12 subunit alpha

UniProt Gene Name

Il12a

UniProt Synonym Gene Names

IL-12A; CLMF p35

UniProt Entry Name

IL12A_MOUSE

Uniprot Description

Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC

By similarity.

Subunit structure: Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12.

Subcellular location: Secreted.

Sequence similarities: Belongs to the IL-6 superfamily.

Research Articles on IL-12

1. These data indicate that IL-12 has a nonprotective role in the acute phase and that IL-12 deficiency results in the accelerated formation of a protective capsule during the healing phase, which is considered crucial for early recovery from disease.

Similar Products

Product Notes

The IL-12 il12a (Catalog #AAA690325) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse IL-12 reacts with Mouse and may cross-react with other species as described in the data sheet. ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 3-4 ug/ml of this antibody will detect at least 0.1 ng/well of recombinant murine IL-12 when used with PeproTech's biotinylated antigen affinity purified anti-murine IL-12 as the detection antibody at a concentration of at least 0.5ug/ml. Researchers should empirically determine the suitability of the IL-12 il12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-12, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Loading...

Rat anti-Mouse IL-12 Monoclonal Antibody | anti-IL-12 antibody