Mouse anti-Human IL-10 Monoclonal Antibody | anti-IL10 antibody

Anti-IL-10 Antibody (Biotin), Mouse Monoclonal

Cat. Num. AAA8118984

• Reactivity: Human
• Applications: ELISA
• Purity: Protein A
• Synonyms: IL-10; Monoclonal Antibody; Anti-IL-10 Antibody (Biotin); Mouse Monoclonal; IL-10 Antibody (Biotin); Mouse MAb; Anti-CSIF Antibody; Anti-GVHDS Antibody; Anti-IL-10 Antibody; Anti-IL10A Antibody; Anti-Interleukin-10 Antibody; Anti-TGIF Antibody; interleukin 10; anti-IL10 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 7
• Specificity: Human IL-10
• Purity/Purification: Protein A
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-IL10 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-1ug/ml
• Immunogen: Recombinant Human IL-10 Protein
• Conjugation: Biotin
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL17A/IL-17A/IL17 (rh IL17A/IL-17A/IL17; Q16552; Ile20-Ala155) and then biotinylated. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and then biotinylated.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Related Product Information for anti-IL10 antibody

Background: IL-10 is an anti-inflammatory cytokine that belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells, and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of some cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen-presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production.

Product Categories/Family for anti-IL10 antibody

Neuroinflammation

References

Arimoto T, et al. (2007) Interleukin-10 protects against inflammation-mediated degeneration of dopaminergic neurons in substantia nigra. Neurobiol Aging. 28(6):894-906.
Han X, et al. (2010) Effect of cobalt protoporphyrin on hyperexpression of heme oxygenase-1 and secretion of IL-10 in rat bone marrow mesenchymal stem cells. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 18(5):1297-301.
Cui QQ, et al. (2011) Expression of RhoA in the lung tissue of acute lung injury rats and the influence of RhoA on the expression of IL-8 and IL-10. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 77(7): 1436-41.