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Mouse anti-Goose IgY Monoclonal Antibody | anti-IgY antibody

MOUSE ANTI DUCK IgY LIGHT CHAIN

Reactivity
Goose
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Synonyms
IgY; Monoclonal Antibody; MOUSE ANTI DUCK IgY LIGHT CHAIN; anti-IgY antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Goose
Clonality
Monoclonal
Isotype
IgG1
Clone Number
14A3
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-IgY antibody
ELISA (EIA), Flow cytometry (FC/FACS), Western Blot (WB)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Duck
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-IgY antibody
Mouse anti Duck IgY Light Chain antibody, clone 14A3 recognizes the light chain of duck immunoglobulin, shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1 (product code MBS212830), revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa (Kothlow et al. 2005). The recognition of B cells by 14A3 was confirmed using a recently developed DYKDDDDK-tagged chicken B cell marker, known as chBAFF-DYKDDDDK (B cell activating factor). Mouse anti Duck IgY Light Chain antibody, clone 14A3 recognizes IgA in Pekin duck bile and can be used as a marker to identify B cells in both Pekin and Muscovy duck and geese studies. Mouse anti Duck IgY Light Chain antibody, clone 14A3 detects a band of approximately 25 kDa in duck cell lysates in Western blotting.

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Product Notes

The IgY (Catalog #AAA212831) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI DUCK IgY LIGHT CHAIN reacts with Goose and may cross-react with other species as described in the data sheet. AAA Biotech's IgY can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow cytometry (FC/FACS), Western Blot (WB). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the IgY for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgY, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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