Flow Cytometry (FC/FACS)
(Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human IgM (CH2) Alexa Fluor 700.)
Mouse anti-Human IgM Monoclonal Antibody | anti-IgM antibody
Anti-Hu IgM Alexa Fluor700
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with Alexa Fluor700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
IgM; Monoclonal Antibody; Anti-Hu IgM Alexa Fluor700; immunoglobulin M; anti-IgM antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
CH2
Specificity
The antibody CH2 reacts with Fc fragment of human IgM.
Purity/Purification
The purified antibody is conjugated with Alexa Fluor700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Concentration
0.1mg/ml (varies by lot)
Applicable Applications for anti-IgM antibody
Flow Cytometry (FC/FACS) (QC tested)
Application Notes
FC/FACS: Recommended dilution: 1-2ug/ml
Conjugation
Alexa Fluor700
Immunogen
Purified human IgM.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Flow Cytometry (FC/FACS)
(Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human IgM (CH2) Alexa Fluor 700.)
Flow Cytometry (FC/FACS)
(Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human IgM (CH2) Alexa Fluor 700.)
Related Product Information for anti-IgM antibody
Immunoglobulin M (IgM) is produced as a 900kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
References
*Franklin EC: Structure and Functional Application (FUNC)tion of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/47690ordinalp,*Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/1122610ordina,*Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/6966908ordina,*Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/8258141ordina
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Product Notes
The IgM (Catalog #AAA1802079) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu IgM Alexa Fluor700 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgM can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS) (QC tested). FC/FACS: Recommended dilution: 1-2ug/ml. Researchers should empirically determine the suitability of the IgM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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