Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Flow Cytometry (FC/FACS) (Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG2a Isotype control (MOPC-173) Alexa Fluor 700 antibody (concentration in sample 9 ug/ml).)

IgG2a isotype control

Mouse IgG2a Isotype Control Alexa Fluor700

Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with Alexa Fluor700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
IgG2a; Monoclonal Isotype Control; Mouse IgG2a Isotype Control Alexa Fluor700; IgG2a isotype control
Ordering
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG2a kappa
Clone Number
MOPC-173
Specificity
This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Purity/Purification
The purified antibody is conjugated with Alexa Fluor700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Concentration
0.1mg/ml (varies by lot)
Applicable Applications for IgG2a isotype control
Flow Cytometry (FC/FACS) (QC tested)
Conjugation
Alexa Fluor700
Negative Species
Mouse, Rat, Human
Immunogen
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Negative Control
The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10ug/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG2a Isotype control (MOPC-173) Alexa Fluor 700 antibody (concentration in sample 9 ug/ml).)

Flow Cytometry (FC/FACS) (Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG2a Isotype control (MOPC-173) Alexa Fluor 700 antibody (concentration in sample 9 ug/ml).)
References
*Fougereau M, Bourgois A, de Preval C, Rocca-Serra J, Schiff C: The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications. Ann Immunol (Paris). 1976 Sep-Oct;127(5):607-31., URL: http://www.ncbi.nlm.nih.gov/pubmed/984731,*Baumal R, Scharff MD: Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone. J Immunol. 1976 Jan;116(1):65-74., URL: http://www.ncbi.nlm.nih.gov/pubmed/812916,*Gupta V, Gylling A, Alonso JL, Sugimori T, Ianakiev P, Xiong JP, Arnaout MA: The beta-tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18. Blood. 2007 Apr 15;109(8):3513-20., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=gupta+v+g,*Khoddami V, Cairns BR: Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP. Nat Protoc. 2014 Feb;9(2):337-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/24434802

Similar Products

Product Notes

The IgG2a (Catalog #AAA1802102) is an Isotype Control produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IgG2a can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS) (QC tested). Researchers should empirically determine the suitability of the IgG2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG2a, Monoclonal Isotype Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.