IgG1 isotype control

Mouse IgG1 Isotype Control Alexa Fluor647

• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: The purified antibody is conjugated with Alexa Fluor647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: IgG1; Monoclonal Isotype Control; Mouse IgG1 Isotype Control Alexa Fluor647; IgG1 isotype control

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: MOPC-21
• Specificity: This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for IgG1 isotype control: Flow Cytometry (FC/FACS) (QC tested), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunocytochemistry (ICC), Western Blot (WB)
• Conjugation: Alexa Fluor647
• Negative Species: Rat, Human
• Negative Control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10ug/ml of incubation mixture), change the conditions of your experiment to reduce the background.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG1 Isotype control (MOPC-21) Alexa Fluor 647 antibody (concentration in sample 9 ug/ml).)

References

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