Canine Prostaglandin E2 ELISA Kit | PGE2 elisa kit

Canine Prostaglandin E2 (PGE2) ELISA Kit

Cat. Num. AAA2603450

• Gene Names: PTGER4; EP4; EP4R
• Reactivity: Canine
• Synonyms: Prostaglandin E2; Canine Prostaglandin E2 (PGE2) ELISA Kit; PGE2 elisa kit

• Reactivity: Canine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 488

• Samples: Canine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 1000 pg/ml-15.6 pg/ml
• Sensitivity: Up to 5 pg/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PGE2 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Canine PGE2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4506259
• NCBI GeneID: 5734
• NCBI Accession #: NP_000949.1
• NCBI GenBank Nucleotide #: NM_000958.2
• Molecular Weight: 53,119 Da
• NCBI Official Full Name: prostaglandin E2 receptor EP4 subtype
• NCBI Official Synonym Full Names: prostaglandin E receptor 4
• NCBI Official Symbol: PTGER4
• NCBI Official Synonym Symbols: EP4; EP4R
• NCBI Protein Information: prostaglandin E2 receptor EP4 subtype
• UniProt Protein Name: Prostaglandin E2 receptor EP4 subtype
• Protein Family: Prostaglandin E2 receptor
• UniProt Gene Name: PTGER4
• UniProt Synonym Gene Names: PTGER2; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype
• UniProt Entry Name: PE2R4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor can activate T-cell factor signaling. It has been shown to mediate PGE2 induced expression of early growth response 1 (EGR1), regulate the level and stability of cyclooxygenase-2 mRNA, and lead to the phosphorylation of glycogen synthase kinase-3. Knockout studies in mice suggest that this receptor may be involved in the neonatal adaptation of circulatory system, osteoporosis, as well as initiation of skin immune responses. [provided by RefSeq, Jul 2008]

Uniprot Description

PTGER4: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: cell soma; integral to membrane; nuclear membrane; plasma membrane

Molecular Function: prostaglandin E receptor activity; protein binding

Biological Process: chemokinesis; female pregnancy; G-protein signaling, coupled to cAMP nucleotide second messenger; immune response; JNK cascade; maternal process involved in parturition; negative regulation of circadian sleep/wake cycle, REM sleep; negative regulation of cytokine secretion; negative regulation of inflammatory response; negative regulation of integrin activation; negative regulation of interleukin-1 alpha secretion; negative regulation of nitric-oxide synthase biosynthetic process; negative regulation of peptide secretion; positive regulation of bone resorption; positive regulation of cAMP biosynthetic process; positive regulation of cell adhesion; positive regulation of cell proliferation; positive regulation of circadian sleep/wake cycle, non-REM sleep; positive regulation of cytokine secretion; positive regulation of inflammatory response; positive regulation of interleukin-8 production; positive regulation of osteoblast differentiation; positive regulation of smooth muscle cell migration; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of stress fiber formation; response to amino acid stimulus; response to drug; response to lipopolysaccharide; response to mechanical stimulus; response to progesterone stimulus; T-helper cell differentiation