anti-Human IGF-1 Clone 6C9-2E5 Monoclonal Antibody
Anti-human Insulin-like Growth Factor 1 Monoclonal Antibody 6C9-2E5
Reactivity
Human
Applications
ELISA, Immunoblot, Western Blot, Western Blot
Synonyms
IGF-1 Clone 6C9-2E5; Monoclonal Antibody; Anti-human Insulin-like Growth Factor 1 Monoclonal Antibody 6C9-2E5; anti-IGF-1 Clone 6C9-2E5 antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
6C9-2E5
Specificity
Polypeptide ::: % Cross Reactivity
hIGF-1 ::: 100
hIGF-1 ::: 100
Form/Format
Supplied as Ascites Fluid (sterile filtered)
Sequence Length
71
Applicable Applications for anti-IGF-1 Clone 6C9-2E5 antibody
Indirect ELISA, Western Immunoblot, Western Blot
Application Notes
ELISA: This monoclonal antibodies containing ascites fluid has been found by indirect ELISA to bind to hIGF-1. Titration experiments show the titer to greater than 1:4000 by indirect ELISA
Western Blot: Western immunoblots resulted in a single band being detected at ~ 7.5 kDa at a dilution of 1:1000
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in 1:5 diluted evaporated goat milk. using TBS/Tween-20 buffer as diluent.
2. Wash x 2 with TBS/Tween-20.
3. Apply the ascites after dilution to at least 1:1000 (Note:higher dilutions may be needed). Use 1% normal goat serum in 1:5 diluted evaporated goat milk as buffer for the primary antibody. Dilute the condensed goat milk with TBS/Tween-20. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-mouse IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 1% normal goat serum in 1:5 diluted evaporated goat milk (useTBS/Tween-20 to dilute the goat milk). Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 3 and then soak the membrane overnight in a fairly large volume of TBS/Tween-20.
7. Develop color using the DAB reaction or the enhanced DAB reaction. FAX: 702-638-7801 www.RDAbs.com e-mail: [email protected]
Western Blot: Western immunoblots resulted in a single band being detected at ~ 7.5 kDa at a dilution of 1:1000
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in 1:5 diluted evaporated goat milk. using TBS/Tween-20 buffer as diluent.
2. Wash x 2 with TBS/Tween-20.
3. Apply the ascites after dilution to at least 1:1000 (Note:higher dilutions may be needed). Use 1% normal goat serum in 1:5 diluted evaporated goat milk as buffer for the primary antibody. Dilute the condensed goat milk with TBS/Tween-20. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-mouse IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 1% normal goat serum in 1:5 diluted evaporated goat milk (useTBS/Tween-20 to dilute the goat milk). Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 3 and then soak the membrane overnight in a fairly large volume of TBS/Tween-20.
7. Develop color using the DAB reaction or the enhanced DAB reaction. FAX: 702-638-7801 www.RDAbs.com e-mail: [email protected]
Related Product Information for anti-IGF-1 Clone 6C9-2E5 antibody
This ascites fluid contains mouse monoclonal antibody 6C9-2E5 raised against recombinant hIGF-1. This monoclonal has been found to stain specifically hIGF-1 in western immunoblots and to bind to hIGF-1 by indirect ELISA. This monoclonal antibody was tested for recognition of Insulin and other growth factors by ELISA techniques. It has been found to be a mouse IgG1 Kappa by isotyping
Product Categories/Family for anti-IGF-1 Clone 6C9-2E5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
IGF-1
Similar Products
Product Notes
The IGF-1 Clone 6C9-2E5 (Catalog #AAA350016) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-human Insulin-like Growth Factor 1 Monoclonal Antibody 6C9-2E5 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IGF-1 Clone 6C9-2E5 can be used in a range of immunoassay formats including, but not limited to, Indirect ELISA, Western Immunoblot, Western Blot. ELISA: This monoclonal antibodies containing ascites fluid has been found by indirect ELISA to bind to hIGF-1. Titration experiments show the titer to greater than 1:4000 by indirect ELISA Western Blot: Western immunoblots resulted in a single band being detected at ~ 7.5 kDa at a dilution of 1:1000 Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in 1:5 diluted evaporated goat milk. using TBS/Tween-20 buffer as diluent. 2. Wash x 2 with TBS/Tween-20. 3. Apply the ascites after dilution to at least 1:1000 (Note:higher dilutions may be needed). Use 1% normal goat serum in 1:5 diluted evaporated goat milk as buffer for the primary antibody. Dilute the condensed goat milk with TBS/Tween-20. Let the primary antibody bind for 2-4 hours. 4. Wash x 3 with TBS/Tween-20. 5. Apply affinity purified HRP-goat anti-mouse IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 1% normal goat serum in 1:5 diluted evaporated goat milk (useTBS/Tween-20 to dilute the goat milk). Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques. 6. Wash x 3 and then soak the membrane overnight in a fairly large volume of TBS/Tween-20. 7. Develop color using the DAB reaction or the enhanced DAB reaction. FAX: 702-638-7801 www.RDAbs.com e-mail: [email protected]. Researchers should empirically determine the suitability of the IGF-1 Clone 6C9-2E5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IGF-1 Clone 6C9-2E5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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