Mouse anti-Human IFNAR2 Monoclonal Antibody | anti-IFNAR2 antibody

Anti-IFNAR2 Antibody (APC), Mouse Monoclonal

Cat. Num. AAA8102458

• Gene Names: IFNAR2; IFN-R; IMD45; IFNABR; IFNARB; IFN-alpha-REC
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: IFNAR2; Monoclonal Antibody; Anti-IFNAR2 Antibody (APC); Mouse Monoclonal; IFNAR2 Antibody (APC); Mouse MAb; interferon (alpha; beta and omega) receptor 2; Anti-IFN-alpha-REC Antibody; Anti-IFN-R Antibody; Anti-IFNABR Antibody; Anti-IFNARB Antibody; anti-IFNAR2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 07
• Specificity: Human IFNAR2
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 10ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-IFNAR2 antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Human IFNAR2 protein
• Conjugation: APC
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IFNAR2 (rh IFNAR2) and conjugated with APC under optimum conditions, the unreacted APC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Testing Data

(Profile of anti-IFNAR2 reactivity on Jurkat cells analyzed by flow cytometry.)

Related Product Information for anti-IFNAR2 antibody

Interferon-alpha/beta receptor beta chain (IFNAR2) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulate Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Initial cell-surface IFNAR2 expression at identification assessed by flow cytometry was widely distributed but showed overall significantly higher expression in CML patients when compared with normal controls. In 15 fresh patients who subsequently received IFNalpha therapy, IFNAR2 expression at identification was significantly higher in cytogenetic good responders than in poor responders. Down-regulation of IFNAR2 expression during IFNalpha therapy was observed only in good responders but not in poor responders. The encoded protein also functions as an antiviral factor. IFNAR2 may associate with IFNAR1 to form the type I interferon receptor. This protein serves as a receptor for interferons alpha and beta. IFNAR2 is also involved in IFN-mediated STAT1, STAT2, and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity. Following binding of IFNalpha2, IFNAR2 is internalized, but, instead of being routed towards degradation as it is when complexed to IFNbeta, it recycles back to the cell surface.

References

Ito K, et al. (2004) Initial expression of interferon alpha receptor 2 (IFNAR2) on CD34-positive cells and its down-regulation correlate with clinical response to interferon therapy in chronic myelogenous leukemia. Eur J Haematol. 73(3): 191-205.Kim SH, et al. (1997) Mammalian type I interferon receptors consists of two subunits: IFNaR1 and IFNaR2. Gene. 196(1-2): 279-86.Saleh AZ, et al. (2004) Regulated proteolysis of the IFNaR2 subunit of the interferon-alpha receptor. Oncogene. 23(42): 7076-86.

NCBI and Uniprot Product Information

• NCBI GI #: 46488937
• NCBI GeneID: 3455
• NCBI Accession #: NP_997468.1
• NCBI GenBank Nucleotide #: NM_207585.2
• UniProt Accession #: P48551; Q15467; Q6FHD7; A8KAJ4; D3DSE8; D3DSE9
• Molecular Weight: 27,384 Da
• NCBI Official Full Name: interferon alpha/beta receptor 2 isoform a
• NCBI Official Synonym Full Names: interferon alpha and beta receptor subunit 2
• NCBI Official Symbol: IFNAR2
• NCBI Official Synonym Symbols: IFN-R; IMD45; IFNABR; IFNARB; IFN-alpha-REC
• NCBI Protein Information: interferon alpha/beta receptor 2
• UniProt Protein Name: Interferon alpha/beta receptor 2
• Protein Family: Interferon alpha/beta receptor
• UniProt Gene Name: IFNAR2
• UniProt Synonym Gene Names: IFNABR; IFNARB; IFN-R-2; IFN-alpha binding protein; IFN-alpha/beta receptor 2
• UniProt Entry Name: INAR2_HUMAN

NCBI Description

The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Multiple transcript variants encoding at least two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

IFNAR2: Associates with IFNAR1 to form the type I interferon receptor. Receptor for interferons alpha and beta. Involved in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity. Belongs to the type II cytokine receptor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: protein binding; interferon-alpha/beta receptor activity; interferon-alpha/beta binding; protein kinase binding

Biological Process: regulation of transcription from RNA polymerase II promoter; cell proliferation; cell surface receptor linked signal transduction; cytokine and chemokine mediated signaling pathway; response to virus; JAK-STAT cascade

Disease: Hepatitis B Virus, Susceptibility To