Rat anti-Mouse IFN-g Monoclonal Antibody | anti-Ifng antibody
Rat Anti-Mouse IFN-g-FITC
Gene Names
Ifng; Ifg; IFN-g
Reactivity
Mouse
Applications
ELISA
Synonyms
IFN-g; Monoclonal Antibody; Rat Anti-Mouse IFN-g-FITC; Rat Anti-Mouse IFN-gamma; anti-Ifng antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
XMG1.2
Specificity
Mouse Interferon-gamma (IFN-gamma) This is a neutralizing antibody
Form/Format
Fluorescein (FITC) Conjugate
Applicable Applications for anti-Ifng antibody
ELISA
Application Notes
ELISA: XMG1.2 monoclonal antibody can be used as a capture antibody in a sandwich ELISA for quantifying mouse IFN-gamma protein levels. Biotinylated XMG1.2 antibody can be paired with a suitable purified capture antibody, with purified recombinant human IFN-gamma as the standard.; Western Blot: The purified XMG1.2 antibody is also useful for Western Blot applications. For Western Blot, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig, has been found to enable detection of <= 100 ng/lane of recombinant mouse IFN-gamma under reducing conditions.; Immunofluorescence/Flow Cytometry: XMG1.2 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IFN-gamma-positive cells within mixed cell populations.4-6 Recombinant mouse IFN-gamma or Rat IgG1 should be used as a specificity control. FITC conjugated XMG1.2 antibodies are useful for intracellular staining in cells producing IFN-gamma. Intracellular staining buffers are recommended for this application.; Neutralization: The LE/AF form of XMG1.2 can be used for neutralizing mouse IFN-gamma bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.; Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Immunogen
E. coli-expressed recombinant mouse IFN-gamma
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
Warning
Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Preparation and Storage
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
References
1. Cherwinski, H.M., J.H. Schumacher, K.D. Brown, and T.R. Mossman. 1987. J. Exp. Med. 166:1229.
2. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5.
3. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. J. Immunol. Meth. 166:201.
4. Vickingson, A., K. Pederson, and D. Muller. 1994. J. Immunol. Meth. 173:219.
5. Ferrick, D.A., M.D. Schrenzel, T. Mulvania, B. Hsieh, W.G. Ferlin, and H. Lepper. 1995. Nature 373:255.
6. Hsieh, B., M. Schrenzel, T. Mulvania, H. Lepper, L. DiMolfetto-Landon, and D. Ferrick. 1996. J. Immunol. 156:232.
2. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5.
3. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. J. Immunol. Meth. 166:201.
4. Vickingson, A., K. Pederson, and D. Muller. 1994. J. Immunol. Meth. 173:219.
5. Ferrick, D.A., M.D. Schrenzel, T. Mulvania, B. Hsieh, W.G. Ferlin, and H. Lepper. 1995. Nature 373:255.
6. Hsieh, B., M. Schrenzel, T. Mulvania, H. Lepper, L. DiMolfetto-Landon, and D. Ferrick. 1996. J. Immunol. 156:232.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,907 Da
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon gamma
NCBI Official Symbol
Ifng
NCBI Official Synonym Symbols
Ifg; IFN-g
NCBI Protein Information
interferon gamma; IFN-gamma; gamma interferon
UniProt Protein Name
Interferon gamma
Protein Family
UniProt Gene Name
Ifng
UniProt Synonym Gene Names
IFN-gamma
UniProt Entry Name
IFNG_MOUSE
NCBI Description
This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]
Uniprot Description
Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Subunit structure: Homodimer.
Subcellular location: Secreted.
Tissue specificity: Released primarily from activated T lymphocytes.
Sequence similarities: Belongs to the type II (or gamma) interferon family.
Research Articles on Ifng
Similar Products
Product Notes
The Ifng ifng (Catalog #AAA670352) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse IFN-g-FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IFN-g can be used in a range of immunoassay formats including, but not limited to, ELISA. ELISA: XMG1.2 monoclonal antibody can be used as a capture antibody in a sandwich ELISA for quantifying mouse IFN-gamma protein levels. Biotinylated XMG1.2 antibody can be paired with a suitable purified capture antibody, with purified recombinant human IFN-gamma as the standard.; Western Blot: The purified XMG1.2 antibody is also useful for Western Blot applications. For Western Blot, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig, has been found to enable detection of <= 100 ng/lane of recombinant mouse IFN-gamma under reducing conditions.; Immunofluorescence/Flow Cytometry: XMG1.2 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IFN-gamma-positive cells within mixed cell populations.4-6 Recombinant mouse IFN-gamma or Rat IgG1 should be used as a specificity control. FITC conjugated XMG1.2 antibodies are useful for intracellular staining in cells producing IFN-gamma. Intracellular staining buffers are recommended for this application.; Neutralization: The LE/AF form of XMG1.2 can be used for neutralizing mouse IFN-gamma bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.; Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. Researchers should empirically determine the suitability of the Ifng ifng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFN-g, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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