Mouse anti-Human IFI16 Monoclonal Antibody | anti-IFI16 antibody

Anti-IFI16 Antibody (clone 4E6)

Cat. Num. AAA2400907

• Gene Names: IFI16; PYHIN2; IFNGIP1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified from ascites by Protein A
• Synonyms: IFI16; Monoclonal Antibody; Anti-IFI16 Antibody (clone 4E6); Mouse Monoclonal [clone 1B4] (IgG2a; k) to Human IFI16; IFNGIP1; Ifi-16; PYHIN2; anti-IFI16 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, k
• Clone Number: 4E6
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4.
• Sequence Length: 729

• Applicable Applications for anti-IFI16 antibody: ELISA (EIA), Immunohistochemistry (IHC) Formalin, Western Blot (WB)
• Application Notes: IHC-Fr: 1:50
• Immunogen: IFI16 (AAH17059, 630 a.a. ~ 730 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.; Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(IHC with IFI16 antibody on frozen human tonsil.)

Western Blot (WB)

(IFI16 monoclonal antibody, clone 4E6 Western blot of IFI16 expression in HeLa NE.)

ELISA (EIA)

(Detection limit for recombinant GST tagged IFI16 is approximately 0.3 ng/ml as a capture antibody.)

Related Product Information for anti-IFI16 antibody

Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have arole in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein.

NCBI and Uniprot Product Information

• NCBI GI #: 112789562
• NCBI GeneID: 3428
• NCBI Accession #: NP_005522.2
• NCBI GenBank Nucleotide #: NM_005531.2
• UniProt Accession #: Q16666; Q59GX0; Q5T3W7; Q5T3W8; Q5T3X0; Q5T3X1; Q5T3X2; Q8N9E5; Q8NEQ7; Q96AJ5; B4DJT8; H3BLV7
• Molecular Weight: 82,588 Da
• NCBI Official Full Name: gamma-interferon-inducible protein 16 isoform 2
• NCBI Official Synonym Full Names: interferon gamma inducible protein 16
• NCBI Official Symbol: IFI16
• NCBI Official Synonym Symbols: PYHIN2; IFNGIP1
• NCBI Protein Information: gamma-interferon-inducible protein 16
• UniProt Protein Name: Gamma-interferon-inducible protein 16
• Protein Family: Gamma-interferon-inducible protein
• UniProt Gene Name: IFI16
• UniProt Synonym Gene Names: IFNGIP1; Ifi-16

NCBI Description

This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]

Uniprot Description

Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors. Necessary to activate the IRF3 signaling cascade during human herpes simplex virus 1 (HHV-1) infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibition of viral immediate-early gene expression and replication. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.