anti-Human IDS Monoclonal Antibody | anti-IDS antibody

Anti-IDS Antibody (clone 3B10) IHC-plus

Cat. Num. AAA249242

• Gene Names: IDS; MPS2; SIDS
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
• Purity: Protein A/G purified
• Synonyms: IDS; Monoclonal Antibody; Anti-IDS Antibody (clone 3B10) IHC-plus; Mouse Monoclonal [clone 3B10] (IgG2a) to Human IDS; Human IDS; Idursulfase; SIDS; Iduronate 2-sulfatase; MPS2; anti-IDS antibody

• Host: IDS/Iduronate 2 Sulfatase antibody was produced in Mouse.
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 3B10
• Specificity: Human IDS
• Purity/Purification: Protein A/G purified
• Form/Format: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA.
• Concentration: 0.79 mg/ml (varies by lot)
• Sequence Length: 550

• Applicable Applications for anti-IDS antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC)
• Application Notes: Flow Cytometry (1:100); IF (1:100); IHC-P (10 ug/ml); WB (1:2000)
• Immunogen: IDS/Iduronate 2 Sulfatase antibody was raised against Human
• Target: IDS/Iduronate 2 Sulfatase.
• Antigen Type: Recombinant Protein
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Store at -20°C. Minimize freezing and thawing.

Immunohistochemistry - Paraffin (IHC)

(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(Anti-IDS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDS.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDS.)

Flow Cytometry (FC/FACS)

(Flow cytometry of HeLa cells, using anti-IDS antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Flow Cytometry (FC/FACS)

(Flow cytometry of Jurkat cells, using anti-IDS antibody (Red), compared to a nonspecific negative control antibody (Blue).)

NCBI and Uniprot Product Information

• NCBI GI #: 4557659
• NCBI GeneID: 3423
• NCBI Accession #: NP_000193.1
• NCBI GenBank Nucleotide #: NM_000202.7
• UniProt Accession #: P22304; Q14604; Q9BRM3; D3DWT4
• NCBI Official Full Name: iduronate 2-sulfatase isoform a preproprotein
• NCBI Official Synonym Full Names: iduronate 2-sulfatase
• NCBI Official Symbol: IDS
• NCBI Official Synonym Symbols: MPS2; SIDS
• NCBI Protein Information: iduronate 2-sulfatase
• UniProt Protein Name: Iduronate 2-sulfatase
• Protein Family: Iduronate 2-sulfatase
• UniProt Gene Name: IDS
• UniProt Synonym Gene Names: SIDS; Idursulfase
• UniProt Entry Name: IDS_HUMAN

NCBI Description

This gene encodes a member of the sulfatase family of proteins. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. This enzyme is involved in the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

IDS: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Defects in IDS are the cause of mucopolysaccharidosis type 2 (MPS2); also known as Hunter syndrome. MPS2 is an X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into adulthood, with attenuated somatic complications and often without mental retardation. Belongs to the sulfatase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.6.13; Hydrolase; Glycan Metabolism - glycosaminoglycan degradation

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: lysosomal lumen

Molecular Function: iduronate-2-sulfatase activity; metal ion binding

Biological Process: carbohydrate metabolic process; chondroitin sulfate catabolic process; chondroitin sulfate metabolic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process

Disease: Mucopolysaccharidosis, Type Ii