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Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant IDS protein (red) or empty vector (blue) stained using IDS antibody)
Mouse IDS Monoclonal Antibody | anti-IDS antibody
IDS antibody
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
IDS antibody was purified by affinity chromatography.
Synonyms
IDS; Monoclonal Antibody; IDS antibody; Monoclonal IDS; Anti-IDS;; anti-IDS antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1D9
Purity/Purification
IDS antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.5-1 mg/ml (varies by lot)
Applicable Applications for anti-IDS antibody
Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100
FC: 1:100
IHC: 1:150
WB: 1:500-2000
FC: 1:100
IHC: 1:150
WB: 1:500-2000
Biological Significance
Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence similarity with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase.
Cross-Reactivity
Human, Mouse
Immunogen
IDS antibody was raised in Mouse using full length human recombinant protein of human IDS produced in HEK293T cell as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant IDS protein (red) or empty vector (blue) stained using IDS antibody)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant IDS protein (red) or empty vector (blue) stained using IDS antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant IDS protein (Right) or empty vector (Left) detected with IDS antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant IDS protein (Right) or empty vector (Left) detected with IDS antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of IDS protein in paraffin embedded Carcinoma of Human liver tissue using IDS antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of IDS protein in paraffin embedded Carcinoma of Human liver tissue using IDS antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant IDS protein using IDS antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant IDS protein using IDS antibody)
Related Product Information for anti-IDS antibody
Mouse monoclonal IDS antibody
Product Categories/Family for anti-IDS antibody
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Product Notes
The IDS (Catalog #AAA833115) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IDS can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100 FC: 1:100 IHC: 1:150 WB: 1:500-2000. Researchers should empirically determine the suitability of the IDS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IDS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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