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Western Blot (WB) (Western Blotting Analysis: Representative lot data. Human lung tissue lysate was probed with ID4 (1ug/ml). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates ID4 (~17kD).)

Mouse anti-Human, Mouse ID4 Monoclonal Antibody | anti-ID4 antibody

ID4 (DNA-binding Protein Inhibitor ID-4, Class B Basic Helix-loop-helix Protein 27, bHLHb27, Inhibitor of DNA Binding 4, BHLHB27) (HRP)

Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
ID4; Monoclonal Antibody; ID4 (DNA-binding Protein Inhibitor ID-4; Class B Basic Helix-loop-helix Protein 27; bHLHb27; Inhibitor of DNA Binding 4; BHLHB27) (HRP); anti-ID4 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
13A200
Specificity
Recognizes human ID4. Species Crossreactivity: Mouse.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-ID4 antibody
Western Blot (WB)
Immunogen
Synthetic linear peptide corresponding to human ID4 (KLH).
Conjugate
HRP
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western Blotting Analysis: Representative lot data. Human lung tissue lysate was probed with ID4 (1ug/ml). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates ID4 (~17kD).)

Western Blot (WB) (Western Blotting Analysis: Representative lot data. Human lung tissue lysate was probed with ID4 (1ug/ml). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates ID4 (~17kD).)

Western Blot (WB)

(Western Blotting Analysis: Representative lot data. Mouse lymph node tissue lysate was probed with ID4 (1ug/ml). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates ID4 (~17kD).)

Western Blot (WB) (Western Blotting Analysis: Representative lot data. Mouse lymph node tissue lysate was probed with ID4 (1ug/ml). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates ID4 (~17kD).)
Product Categories/Family for anti-ID4 antibody
References
1. Ying, Q. L., et al. (2003). Cell. 115:281-292.

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
NCBI Official Full Name
ID4, partial
UniProt Protein Name
DNA-binding protein inhibitor ID-4
UniProt Gene Name
ID4
UniProt Synonym Gene Names
BHLHB27; bHLHb27
UniProt Entry Name
ID4_HUMAN

NCBI Description

This gene encodes a member of the inhibitor of DNA binding (ID) protein family. These proteins are basic helix-loop-helix transcription factors which can act as tumor suppressors but lack DNA binding activity. Consequently, the activity of the encoded protein depends on the protein binding partner. [provided by RefSeq, Dec 2011]

Uniprot Description

ID4: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; protein binding; transcription corepressor activity

Biological Process: fat cell differentiation; circadian rhythm; transcription, DNA-dependent; myelination in the central nervous system; hippocampus development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; cerebral cortex neuron differentiation; regulation of transcription from RNA polymerase II promoter; negative regulation of oligodendrocyte differentiation; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; positive regulation of cell proliferation; negative regulation of astrocyte differentiation; neuroblast proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle

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Product Notes

The ID4 id4 (Catalog #AAA6244124) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ID4 (DNA-binding Protein Inhibitor ID-4, Class B Basic Helix-loop-helix Protein 27, bHLHb27, Inhibitor of DNA Binding 4, BHLHB27) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ID4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the ID4 id4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ID4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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