Testing Data
(Jurkat T cells that expressed high (ICAM-3 high= red) or low (ICAM-3 low= blue) levels of ICAM-3 were stained with mAb BH10. 5 x 10^5 cells were stained with 100l of mAb. Following 30 min incubation at 4 degree C, unbound Ab was removed by washing and bound mAb detected by staining with goat antimouse- FITC for 30 min at 4 degree C. Flow cytometric histogram of 5000 events per sample is shown.
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Mouse anti-Human ICAM-3 Monoclonal Antibody | anti-ICAM-3 antibody
Mouse anti ICAM-3
Testing Data
(Jurkat T cells that expressed high (ICAM-3 high= red) or low (ICAM-3 low= blue) levels of ICAM-3 were stained with mAb BH10. 5 x 10^5 cells were stained with 100l of mAb. Following 30 min incubation at 4 degree C, unbound Ab was removed by washing and bound mAb detected by staining with goat antimouse- FITC for 30 min at 4 degree C. Flow cytometric histogram of 5000 events per sample is shown. )
Testing Data
(Jurkat T cells that expressed high (ICAM-3 high= red) or low (ICAM-3 low= blue) levels of ICAM-3 were stained with mAb BH10. 5 x 10^5 cells were stained with 100l of mAb. Following 30 min incubation at 4 degree C, unbound Ab was removed by washing and bound mAb detected by staining with goat antimouse- FITC for 30 min at 4 degree C. Flow cytometric histogram of 5000 events per sample is shown.
)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [provided by RefSeq, Jul 2008]
Uniprot Description
ICAM3: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. Belongs to the immunoglobulin superfamily. ICAM family.
Protein type: Membrane protein, integral; Immunoglobulin superfamily; Cell adhesion
Chromosomal Location of Human Ortholog: 19p13.3-p13.2
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: integrin binding; protein binding; receptor binding
Biological Process: cell-cell adhesion; extracellular matrix organization and biogenesis; regulation of immune response; cell adhesion
Research Articles on ICAM-3
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Product Notes
The ICAM-3 icam3 (Catalog #AAA570340) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti ICAM-3 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ICAM-3 can be used in a range of immunoassay formats including, but not limited to, Enzyme Linked Immuno Sorbent Assay, Flow Cytometry (FC/FACS), Immunohistochemistry on frozen and paraffin sections, Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Immuno-cryo-electron microscopy. BH10 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications. Researchers should empirically determine the suitability of the ICAM-3 icam3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICAM-3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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