Mouse Hydroxyguanosine 8 Monoclonal Antibody
Hydroxyguanosine 8 (8-OHG)
Applications
ELISA, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Hydroxyguanosine 8; Monoclonal Antibody; Hydroxyguanosine 8 (8-OHG); Anti -Hydroxyguanosine 8 (8-OHG); anti-Hydroxyguanosine 8 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
9L175
Specificity
Recognizes 8-Hydroxyguanosine. Crossreactivity: 8-hydroxy-2'-deoxyguanosine (oh8dG), 8-hydroxyguanine (oh8G), and 8-hydroxyguanosine (oh8G).
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in TBS, pH 7.4, 0.1% sodium azide.
Applicable Applications for anti-Hydroxyguanosine 8 antibody
ELISA (EL/EIA), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA and Immunohistochemistry (PLP-fixed sections).
Immunogen
8-hydroxyguanosine (oh8G)-BSA and -casein conjugates.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Hydroxyguanosine 8 antibody
Reactive oxygen species (ROS) are formed at high levels as by-products of the normal cellular metabolism. Both nuclear and mitochondrial DNA has been shown to accumulate high levels of 8-hydroxy-2'-deoxyguanosine, a very stable and damaging product of hydroxylation of guanine at carbon 8. 8-hydroxyguanosine (8-OHG) induces transversion of G to T, which is potentially mutagenic. The base excision repair (BER) pathway is the most important cellular protection mechanism responding to oxidative DNA damage. They remove modified DNA bases before they are incorporated into DNA during replication. The key enzymes MutT homologs (MutT/MTH) in the BER process are DNA glycosylases, which remove different damaged bases by cleavage of the N-glycosylic bonds between the bases and the deoxyribose moieties of the nucleotide residues. The 8-oxoG glycosylases (Fpg or MutM/OGG) and the MutY homologs (MutY/MYH) glycosylases along with MutT/MTH protect cells from the mutagenic effects of 8-oxoG. 8-hydroxyguanosine (8-OHG) has been used as oxidative stress marker.
Product Categories/Family for anti-Hydroxyguanosine 8 antibody
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Product Notes
The Hydroxyguanosine 8 (Catalog #AAA605273) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hydroxyguanosine 8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC). Suitable for use in ELISA and Immunohistochemistry (PLP-fixed sections). Researchers should empirically determine the suitability of the Hydroxyguanosine 8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hydroxyguanosine 8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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