Western Blot (WB)
(The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HTATIP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate )
Mouse anti-Human HTATIP2 Monoclonal Antibody | anti-HTATIP2 antibody
HTATIP2 antibody
Gene Names
HTATIP2; CC3; TIP30; SDR44U1
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
HTATIP2; Monoclonal Antibody; HTATIP2 antibody; Oxidoreductase HTATP2; CC3; TIP30; SDR44u1; HTATIP 2; HTATIP-2; CC-3; TIP 30; TIP-30; anti-HTATIP2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT5D6
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
242
Applicable Applications for anti-HTATIP2 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human HTATIP2 (1-242aa) purified from E. coli
Clone Note
Anti-human HTATIP2 mAb, clone AT5D6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HTATIP2 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HTATIP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate )
Western Blot (WB)
(The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HTATIP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate )
Immunofluorescence (IF)
(ICC/IF analysis of HTATIP2 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HTATIP2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of HTATIP2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HTATIP2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of HTATIP2 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HTATIP2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of HTATIP2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HTATIP2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-HTATIP2 antibody
HTATIP2, also known as TIP30, is a member of the short-chain dehydrogenases/reductases (SDR) family. It acts as a tumor suppressor participating in metabolic suppression, inhibition of angiogenesis and induces the expression of apoptosis related genes Bad and Siva. HTATIP2 interacts with the activation domain of HIV-1 TAT and enhances its transcription by phosphorylating RNA polymerase II (Pol II). Defects in This protein are associated with hepatocellular carcinomas and apoptotic resistant tumor cells, suggesting a potential use for HTATIP2 in antitumor therapy.
Product Categories/Family for anti-HTATIP2 antibody
References
Shtivelman E., et al. (1997) Oncogene. 14 (18): 2167-73. ; Xiao H., et al. (1998) Proc Natl Acad Sci u S A. 95 (5): 2146-2151.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,131 Da
NCBI Official Full Name
HTATIP2 protein
NCBI Official Synonym Full Names
HIV-1 Tat interactive protein 2
NCBI Official Symbol
HTATIP2
NCBI Official Synonym Symbols
CC3; TIP30; SDR44U1
NCBI Protein Information
oxidoreductase HTATIP2
UniProt Protein Name
Oxidoreductase HTATIP2
Protein Family
UniProt Gene Name
HTATIP2
Uniprot Description
Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.
Research Articles on HTATIP2
Similar Products
Product Notes
The HTATIP2 htatip2 (Catalog #AAA200450) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HTATIP2 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HTATIP2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the HTATIP2 htatip2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HTATIP2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
