Mouse HSP70 Monoclonal Antibody | anti-HSP70 antibody

HSP70 Antibody

Cat. Num. AAA8001873

• Reactivity: Human, Mouse, Rat, Bovine, Pig
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, ELISA
• Purity: Protein G Purified
• Synonyms: HSP70; Monoclonal Antibody; HSP70 Antibody; Mouse Anti-Bovine HSP70 Monoclonal IgG1; Clone 1.86; HSP70 1 Antibody; HSP70 2 Antibody; HSP70.1 Antibody; HSP72 Antibody; HSPA1 Antibody; HSPA1A Antibody; HSPA1B Antibody; anti-HSP70 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Bovine, Pig
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1.86
• Specificity: Detects 70kDa. Does not cross react with HSC70.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-HSP70 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500; ICC/IF: 1:100
• Immunogen: Bovine HSP70
• Immunogen Species: Bovine
• Conjugate: Unconjugated
• Cellular Localization: Cytoplasm
• Certificate of Analysis: 1 ug/ml of SMC-113 was sufficient for detection of HSP70 in 20 ug of Hela lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Preparation and Storage: Store at-20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1.86 . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.)

Western Blot (WB)

(Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293) lysates showing detection of ~70 kDa Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 1.86 . Lane 1: MW ladder. Lane 2: Human HEK293 lysate (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.)

Related Product Information for anti-HSP70 antibody

HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Product Categories/Family for anti-HSP70 antibody

Cell Signaling; Protein Trafficking; Chaperone Proteins; Cancer; Tumor Biomarkers; Cancer; Heat Shock

References

1. Welch W.J. and Suhan J.P. (1986) J Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J.Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell 59: 591-601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad.Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.

NCBI and Uniprot Product Information

• NCBI GI #: 40254806
• NCBI GeneID: 281825
• NCBI Accession #: NP_776975.1
• NCBI GenBank Nucleotide #: NM_174550.1
• UniProt Accession #: Q27975
• UniProt Protein Name: Heat shock 70 kDa protein 1A
• Protein Family: HSP70 co-chaperone
• UniProt Gene Name: HSPA1A
• UniProt Synonym Gene Names: HSP70-1; HSP70.1

Uniprot Description

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation.