Western Blot (WB)
(Western Blot analysis of Yeast Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 1G10.H8. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Mouse Hsp40 (YDJ1) Monoclonal Antibody | anti-YDJ1 antibody
Hsp40, YDJ1 Antibody: ATTO 594
Gene Names
YDJ1; HSP40; MAB3; MAS5
Reactivity
Yeast. Does not cross react with Human, Mouse or Rat.
Applications
Western Blot, Immunoprecipitation, ELISA
Synonyms
Hsp40 (YDJ1); Monoclonal Antibody; Hsp40; YDJ1 Antibody: ATTO 594; DNAJA2; CPR3; HIRIP4; DNAJ; DNJ3; DJ3; RDJ2; HIRa interatcting protein4; anti-YDJ1 antibody
Host
Mouse
Reactivity
Yeast. Does not cross react with Human, Mouse or Rat.
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
1G10.H8
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
409
Applicable Applications for anti-YDJ1 antibody
Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
Application Notes
1:2000 (WB)
Conjugate
ATTO 594
Storage Buffer
50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western Blot analysis of Yeast Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 1G10.H8. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Western Blot (WB)
(Western Blot analysis of Yeast Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 1G10.H8. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Related Product Information for anti-YDJ1 antibody
Background Info: Detects ~40kDa.
Scientific Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of Hsp40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins (5, 6). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).
Scientific Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of Hsp40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins (5, 6). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).
Product Categories/Family for anti-YDJ1 antibody
References
1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28-36. 2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309-316. 3. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095. 4. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830. 5. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874-2878. 6. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927-20931. 7. Höhfeld J., et al. (2001) EMBO Rep. 2: 885-890.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,671 Da
NCBI Official Full Name
type I HSP40 co-chaperone YDJ1
NCBI Official Symbol
YDJ1
NCBI Official Synonym Symbols
HSP40; MAB3; MAS5
NCBI Protein Information
type I HSP40 co-chaperone YDJ1
UniProt Protein Name
Mitochondrial protein import protein MAS5
UniProt Gene Name
YDJ1
UniProt Synonym Gene Names
MAS5; YNL064C; N2418; YNL2418C
UniProt Entry Name
MAS5_YEAST
Uniprot Description
Probably involved in mitochondrial protein import. Is also required for efficient translocation of pre-pro-alpha-factor. Involved in heme regulation of HAP1, as a component of the high-molecular-weight (HMC) complex.
Research Articles on YDJ1
Similar Products
Product Notes
The YDJ1 ydj1 (Catalog #AAA802364) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hsp40, YDJ1 Antibody: ATTO 594 reacts with Yeast. Does not cross react with Human, Mouse or Rat. and may cross-react with other species as described in the data sheet. AAA Biotech's Hsp40 (YDJ1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA). 1:2000 (WB). Researchers should empirically determine the suitability of the YDJ1 ydj1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp40 (YDJ1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
