Testing Data
(Western blot of human lysates showing detection of ~85 and 95 kDa HSF1 protein using Anti-HSF1, clone 4B4 . Lane 1: MW ladder. Lane 2: A431 lysate, 20 µg. Lane3: HEK293 lysate, 20µg. Block: 5% milk + TBST 1hr at RT. Primary antibody: Rat monoclonal Anti-HSF1, clone 4B4 incubated for 60 min at RT. Secondary antibody: Goat Anti-Rat HRP antibody (1:1000) for 60 min at RT. Colour Development: TMB solution for 5 min at RT. Predicted/Observed size: ~85 and 95 kDa. Other band(s): None.)
Rat HSF1 Monoclonal Antibody | anti-Hsf1 antibody
HSF1 Antibody: ATTO 488
Testing Data
(Western blot of human lysates showing detection of ~85 and 95 kDa HSF1 protein using Anti-HSF1, clone 4B4 . Lane 1: MW ladder. Lane 2: A431 lysate, 20 µg. Lane3: HEK293 lysate, 20µg. Block: 5% milk + TBST 1hr at RT. Primary antibody: Rat monoclonal Anti-HSF1, clone 4B4 incubated for 60 min at RT. Secondary antibody: Goat Anti-Rat HRP antibody (1:1000) for 60 min at RT. Colour Development: TMB solution for 5 min at RT. Predicted/Observed size: ~85 and 95 kDa. Other band(s): None.)
Testing Data
(Western blot of human lysates showing detection of ~85 and 95 kDa HSF1 protein using Anti-HSF1, clone 4B4 . Lane 1: MW ladder. Lane 2: A431 lysate, 20 µg. Lane3: HEK293 lysate, 20µg. Block: 5% milk + TBST 1hr at RT. Primary antibody: Rat monoclonal Anti-HSF1, clone 4B4 incubated for 60 min at RT. Secondary antibody: Goat Anti-Rat HRP antibody (1:1000) for 60 min at RT. Colour Development: TMB solution for 5 min at RT. Predicted/Observed size: ~85 and 95 kDa. Other band(s): None.)
Scientific Background: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
NCBI and Uniprot Product Information
Uniprot Description
HSF1: a transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription. Induced in response to heat, heavy metals, and oxidative stress. In higher eukaryotes, HSF is unable to bind to HSEs unless the cells are stressed. Becomes phosphorylated in response to stress, forming homotrimers that bind DNA and activate transcription. Phosphorylation by PLK1 enhances nuclear translocation, and phosphorylation by CaMKII enhances transactivation. Phosphorylation by GSK3 and ERK1 induces binding by 14-3-3 and sequestration in the cytoplasm. In addition, during attenuation from the heat shock response, HSF1 is repressed by direct binding of Hsp70, HSP40, and HSF binding protein 1 (HSBP1). Four alternatively spliced isoforms have been described.
Protein type: Transcription factor; DNA-binding
Cellular Component: nucleoplasm; protein complex; pronucleus; cytoplasm; nucleus
Molecular Function: DNA binding; sequence-specific DNA binding; chromatin binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; embryonic placenta development; transcription, DNA-dependent; in utero embryonic development; embryonic process involved in female pregnancy; female meiosis; defense response; response to lipopolysaccharide; positive regulation of multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; protein amino acid phosphorylation; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; response to heat; mRNA transcription; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis
Research Articles on Hsf1
Similar Products
Product Notes
The Hsf1 hsf1 (Catalog #AAA804246) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The HSF1 Antibody: ATTO 488 reacts with Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's HSF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), ELISA (EIA), Gel Mobility Shift Assay. 1:1000 WB. Researchers should empirically determine the suitability of the Hsf1 hsf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
