Mouse Hsc70/Hsp 73 Monoclonal Antibody | anti-Hsc70/Hsp 73 antibody
Anti-Hsc70/Hsp73 Monoclonal Antibody
Reactivity
Human, mouse, rat
Applications
ELISA, Immunoblot, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
Hsc70/Hsp 73; Monoclonal Antibody; Anti-Hsc70/Hsp73 Monoclonal Antibody; anti-Hsc70/Hsp 73 antibody
Host
Mouse
Reactivity
Human, mouse, rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1F2-H5
Specificity
Hsc70/Hsp 73
This antibody recognizes human, mouse, and rat Hsc70. Other species have not been tested. It does not cross-react with Hsp70.
This antibody recognizes human, mouse, and rat Hsc70. Other species have not been tested. It does not cross-react with Hsp70.
Form/Format
100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
Applicable Applications for anti-Hsc70/Hsp 73 antibody
ELISA; Immunoblot; Immunohistochemistry; Immunoprecipitation; Immunoprecipitation
Application Notes
Immunoblotting: use at 1ug/ml. A band of ~73kDa is detected. ELISA: use at 1-10ug/ml. Immunohistochemistry: use at 1-10ug/ml. Immunoprecipitation: 1-10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate.
Antigen
Human Hsc70
Preservative
None. Available on request.
Dilution Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
Related Product Information for anti-Hsc70/Hsp 73 antibody
Hsp70 genes, members of a multigene family, encode heat-inducible 70kDa heatshock proteins that function as molecular chaperones. They have been identified in most organelles of eukaryotic cells as well as in bacteria. The N-terminus of Hsp70 binds ATP with high affinity and the Cterminus binds proteins and polypeptides. When cells are subjected to metabolic stress, Hsp70 is expressed. Hsp70 shares >90% sequence homology with Hsc70. Constitutively expressed Hsc70 forms a stable complex with the highly inducible Hsp70 in cells following heat shock. This interaction is regulated by ATP. Research on Hsc70 suggests that it plays a role in facilitating the recovery of centrosomal structure and function after heat shock.
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Product Notes
The Hsc70/Hsp 73 (Catalog #AAA190514) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hsc70/Hsp73 Monoclonal Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Hsc70/Hsp 73 can be used in a range of immunoassay formats including, but not limited to, ELISA; Immunoblot; Immunohistochemistry; Immunoprecipitation; Immunoprecipitation. Immunoblotting: use at 1ug/ml. A band of ~73kDa is detected. ELISA: use at 1-10ug/ml. Immunohistochemistry: use at 1-10ug/ml. Immunoprecipitation: 1-10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate. Researchers should empirically determine the suitability of the Hsc70/Hsp 73 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsc70/Hsp 73, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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