Mouse HSBP1 Monoclonal Antibody | anti-HSBP1 antibody

HSBP1 Antibody

Cat. Num. AAA415118

• Gene Names: HSBP1; NPC-A-13
• Reactivity: Human, cow, pig, mouse, rat and other mammals
• Applications: Immunofluorescence, Western Blot
• Purity: Affinity purified
• Synonyms: HSBP1; Monoclonal Antibody; HSBP1 Antibody; anti-HSBP1 antibody

• Host: Mouse
• Reactivity: Human, cow, pig, mouse, rat and other mammals
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 76

• Applicable Applications for anti-HSBP1 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: The antibody solution can be used at dilutions of at least 1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10,000 or lower.
• Immunogen: The heat shock proteins were discovered, as the name suggests, since they are heavily upregulated when cells are stressed by temperatures above the normal physiological range. They are expressed in unstressed cells also and have a normal function as chaperones, helping other proteins to fold correctly, and are required in much greater amounts if the cell or tissue is stressed by heat. The increased levels are generated transcriptionally under the influence of a powerful transcription factor, the heat shock factor 1 (HSF1). The different heat shock proteins were originally named based on their SDS-PAGE mobility, so HSP27 has an an apparent molecular weight of 27kDa. It is an abundant protein even under non-stress conditions and frequently shows up as a major spot on 2 dimensional gels of cells or tissues. It is known to associate with a variety of other proteins such as actin, intermediate filament subunits and ubiquitin and is found both in the cytoplasm and the nucleus of cells. HSP27 can become heavily phosphorylated under the influence of multiple protein kinases particularly as a result of activation of the p38/SAPK pathway. Upregulation of this protein is protective against neurodegenerative diseases at least in certain mouse models (1). Point mutations in the HSP27 gene are associated with two neurological diseases, Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuropathy IIB (2). These diseases are associated with axonal loss apparently following defects in the transport of neurofilaments. The HGNC name for this protein is HSBP1.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

References

1. Wyttenbach, A et al. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin. Hum. Molec. Genet. 11:1137-1151 (2002).2. Evgrafov, OV et al. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Nature Genet. 36:602-606 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4557647
• NCBI GeneID: 3281
• NCBI Accession #: NP_001528.1
• NCBI GenBank Nucleotide #: NM_001537.3
• UniProt Accession #: O75506; Q53XA8; Q7Z5Z3
• Molecular Weight: 8,544 Da
• NCBI Official Full Name: heat shock factor-binding protein 1
• NCBI Official Synonym Full Names: heat shock factor binding protein 1
• NCBI Official Symbol: HSBP1
• NCBI Official Synonym Symbols: NPC-A-13
• NCBI Protein Information: heat shock factor-binding protein 1; nasopharyngeal carcinoma-associated antigen 13
• UniProt Protein Name: Heat shock factor-binding protein 1
• Protein Family: Heat shock factor-binding protein
• UniProt Gene Name: HSBP1
• UniProt Synonym Gene Names: HSF1BP; NPC-A-13
• UniProt Entry Name: HSBP1_HUMAN

NCBI Description

The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1(HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Negative regulator of the heat shock response. Negatively affects HSF1 DNA-binding activity. May have a role in the suppression of the activation of the stress response during the aging process.

Subunit structure: Homohexamer. Associates with heptad repeats of HSF1 trimers and problaby also HSF1 monomers, and with HSP70. Association with HSF1 trimers and HSP70 coincides with attenuation of heat shock response and the conversion of HSF1 trimer to monomer. Ref.6

Subcellular location: Nucleus.

Sequence similarities: Belongs to the HSBP1 family.