Western Blot (WB)
(Western blot analysis of HRS expression in brain (1) and HeLa cells Iysate (2) using the anti-HRS antibody)
Mouse HRS Monoclonal Antibody | anti-HRS antibody
Hrs (HGS) Mouse Monoclonal Antibody (19-5)
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Synonyms
HRS; Monoclonal Antibody; Hrs (HGS) Mouse Monoclonal Antibody (19-5); Hepatocyte growth factor-regulated tyrosine kinase substrate; HGS; Protein pp110; anti-HRS antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Monoclonal
Isotype
lgG2b
Clone Number
19-5
Specificity
The antibody recognizes an epitope is located between the VHS and coiled-coil domain of the HRS protein.
Form/Format
Lyophilized protein G purified in PBS pH7.4
Applicable Applications for anti-HRS antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
If reconstituted in 50 muL: IHC: 1:300; ICC: 1:200; WB: 1:1000
Immunogen
Full length hrs-2 fusion protein
Preservative
None
Preparation and Storage
Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of HRS expression in brain (1) and HeLa cells Iysate (2) using the anti-HRS antibody)
Western Blot (WB)
(Western blot analysis of HRS expression in brain (1) and HeLa cells Iysate (2) using the anti-HRS antibody)
Related Product Information for anti-HRS antibody
Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS, also known as HGS) is a mammalian protein predominantly localized on early endosomes. Hrs interacts with many proteins involved in membrane trafficking, such as eps15, SfdMdlSNAP-25. Hrs is implicated in early endosome fusion and may play a role in cargo sorting/trafficking to early and late endosome by recruiting clathrin. Hrs is also involved in intracellular signal transduction mediated by cytokines and growth factors.
References
1. Ronning SB-CIN85 regulates ubiquitination and degradative endosomal sorting of the EGF receptor. 2. Pullan L-The endosome-associated protein Hrs is hexameric and controls cargo sorting as a "master molecule". 3. Tsujimoto S-The cellular and developmental expression of hrs-2 in rat.
Similar Products
Product Notes
The HRS (Catalog #AAA588094) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hrs (HGS) Mouse Monoclonal Antibody (19-5) reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's HRS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). If reconstituted in 50 muL: IHC: 1:300; ICC: 1:200; WB: 1:1000. Researchers should empirically determine the suitability of the HRS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
