Western Blot (WB)
(Western blot analysis on K562 and Jurkat cell lysate using CLIM1 Antibody)
CLIM1 Antibody | anti-CLIM1 antibody
CLIM1 Antibody
Gene Names
PDLIM1; CLIM1; CLP36; CLP-36; hCLIM1; HEL-S-112
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity-chromatography
Synonyms
CLIM1; CLIM1 Antibody; C-terminal LIM domain protein 1; carboxy terminal LIM domain protein 1; carboxyl terminal LIM domain protein 1; CLP-36; CLP36; Elfin; enigma; hCLIM1; LIM domain protein CLP-36; PDLI1; PDLIM1; PDZ and LIM domain 1; PDZ and LIM domain protein 1; anti-CLIM1 antibody
Reactivity
Human, Mouse, Rat
Specificity
CLIM1 antibody detects endogenous levels of total CLIM1
Purity/Purification
Affinity-chromatography
Form/Format
In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
329
Applicable Applications for anti-CLIM1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500~1:3000
IHC: 1:50~1:200
IF/ICC: 1:100~1:500
IHC: 1:50~1:200
IF/ICC: 1:100~1:500
Immunogen
A synthesized peptide derived from human CLIM1
Preparation and Storage
Store at-20 degree C for 1 year
Western Blot (WB)
(Western blot analysis on K562 and Jurkat cell lysate using CLIM1 Antibody)
Western Blot (WB)
(Western blot analysis on K562 and Jurkat cell lysate using CLIM1 Antibody)
Related Product Information for anti-CLIM1 antibody
CLIM1 a cytoskeletal adaptor protein that bring other proteins to the cytoskeleton. Interacts with alpha-actinins 1, 2 and 4. Recruits the Clik1 kinase to actin stress fibers in nonmuscle cells. Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36kDa
NCBI Official Full Name
PDZ and LIM domain protein 1
NCBI Official Synonym Full Names
PDZ and LIM domain 1
NCBI Official Symbol
PDLIM1
NCBI Official Synonym Symbols
CLIM1; CLP36; CLP-36; hCLIM1; HEL-S-112
NCBI Protein Information
PDZ and LIM domain protein 1
UniProt Protein Name
PDZ and LIM domain protein 1
UniProt Gene Name
PDLIM1
UniProt Synonym Gene Names
CLIM1; CLP36
NCBI Description
This gene encodes a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. [provided by RefSeq, Oct 2012]
Uniprot Description
Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (PubMed:10861853). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts ().
Research Articles on CLIM1
Similar Products
Product Notes
The CLIM1 pdlim1 (Catalog #AAA8510795) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The CLIM1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CLIM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500~1:3000 IHC: 1:50~1:200 IF/ICC: 1:100~1:500. Researchers should empirically determine the suitability of the CLIM1 pdlim1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLIM1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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