Mouse anti-Human HNRNP-E2 / PCBP2 Monoclonal Antibody | anti-HNRNP-E2 antibody

Mouse Monoclonal [clone 5F12] (IgG2a,k) to Human HNRNP-E2 / PCBP2

Cat. Num. AAA245985

• Gene Names: PCBP2; HNRPE2; HNRNPE2; hnRNP-E2
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: HNRNP-E2 / PCBP2; Monoclonal Antibody; Mouse Monoclonal [clone 5F12] (IgG2a; k) to Human HNRNP-E2 / PCBP2; Anti-HNRNP-E2 / PCBP2 Antibody (clone 5F12) IHC-plus; PCBP2; Alpha-CP2; HnRNP E2; HNRPE2; Poly(rC) binding protein 2; Poly(rC)-binding protein 2; HnRNP-E2; Human HNRNP-E2; anti-HNRNP-E2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 5F12
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 366

• Applicable Applications for anti-HNRNP-E2 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA), RNA interference
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), RNAi, WB; Usage: Western Blot (Cell lysate) - positive control K-562. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein). RNAi Knockdown (A...
• Target Species: Human
• Immunogen Description: PCBP2 (AAH01155, 1 a.a. ~ 363 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: HNRNP-E2 / PCBP2 antibody was raised against pCBP2 (AAH01155, 1 a.a. ~ 363 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-PCBP2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-PCBP2 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PCBP2 on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(Western blot of PCBP2 expression in transfected 293T cell line by PCBP2 monoclonal antibody clone 5F12.)

Western Blot (WB)

(PCBP2 monoclonal antibody clone 5F12 Western blot of PCBP2 expression in K-562.)

RNA interference

(Western blot of PCBP2 over-expressed 293 cell line, cotransfected with PCBP2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PCBP2 monoclonal antibody clone 5F12. GAPDH (36.1 kD) used as specificity.)

ELISA (EIA)

(Detection limit for recombinant GST tagged PCBP2 is approximately 0.1 ng/ml as a capture antibody.)

Related Product Information for anti-HNRNP-E2 antibody

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding.

NCBI and Uniprot Product Information

• NCBI GI #: 14141168
• NCBI GeneID: 5094
• NCBI Accession #: NP_005007.2
• NCBI GenBank Nucleotide #: NM_005016.5
• UniProt Accession #: Q15366; Q32Q82; Q59HD4; Q68Y55; Q6IPF4; Q6PKG5; A8K7X6; F8VYL7; G3V0E8; I6L8F9
• Molecular Weight: 33,497 Da
• NCBI Official Full Name: poly(rC)-binding protein 2 isoform a
• NCBI Official Synonym Full Names: poly(rC) binding protein 2
• NCBI Official Symbol: PCBP2
• NCBI Official Synonym Symbols: HNRPE2; HNRNPE2; hnRNP-E2
• NCBI Protein Information: poly(rC)-binding protein 2; poly(rC)-binding protein 2; hnRNP E2; alpha-CP2; heterogenous nuclear ribonucleoprotein E2; heterogeneous nuclear ribonucleoprotein E2
• UniProt Protein Name: Poly(rC)-binding protein 2
• UniProt Gene Name: PCBP2
• UniProt Synonym Gene Names: hnRNP E2
• UniProt Entry Name: PCBP2_HUMAN

NCBI Description

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP E2: Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: nucleoplasm; focal adhesion; membrane; cytoplasm; nucleus; ribonucleoprotein complex; cytosol

Molecular Function: protein binding; enzyme binding; DNA binding; RNA binding; ubiquitin protein ligase binding

Biological Process: nuclear mRNA splicing, via spliceosome; proteasomal ubiquitin-dependent protein catabolic process; RNA splicing; innate immune response; gene expression; negative regulation of defense response to virus; defense response to virus; negative regulation of interferon type I production; mRNA metabolic process