Rabbit PCBP2/hnRNP E2 Monoclonal Antibody | anti-PCBP2 antibody

PCBP2/hnRNP E2 Rabbit mAb

Cat. Num. AAA4752942

• Gene Names: PCBP2; HNRPE2; HNRNPE2; hnRNP-E2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: PCBP2/hnRNP E2; Monoclonal Antibody; PCBP2/hnRNP E2 Rabbit mAb; Poly(rC)-binding protein 2; HNRPE2; HNRNPE2; hnRNP-E2; anti-PCBP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: R01-2F5
• Specificity: Detected in all tissues examined. Gene expression databases
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-PCBP2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000; ICC/IF: 1:100; FC/FACS: 1:50; IP: 1:20
• Immunogen: A synthetic peptide of human PCBP2
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Cytoplasm, Nucleus
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of PCBP2/hnRNP E2 in C6,3T3 cell lysates using PCBP2/hnRNP E2 Rabbit mAb(1:1000 diluted). Predicted band size:39kDa. Observed band size: 35-45kDa.)

Related Product Information for anti-PCBP2 antibody

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2018]
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling (PubMed:19881509). It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitination and degradation (PubMed:19881509).

NCBI and Uniprot Product Information

• NCBI GI #: 6707736
• NCBI GeneID: 5094
• UniProt Accession #: Q15366; Q32Q82; Q59HD4; Q68Y55; Q6IPF4; Q6PKG5; A8K7X6; F8VYL7; G3V0E8; I6L8F9
• Molecular Weight: 365
• NCBI Official Full Name: Poly(rC)-binding protein 2
• NCBI Official Synonym Full Names: poly(rC) binding protein 2
• NCBI Official Symbol: PCBP2
• NCBI Official Synonym Symbols: HNRPE2; HNRNPE2; hnRNP-E2
• NCBI Protein Information: poly(rC)-binding protein 2; poly(rC)-binding protein 2; hnRNP E2; alpha-CP2; heterogenous nuclear ribonucleoprotein E2; heterogeneous nuclear ribonucleoprotein E2
• UniProt Protein Name: Poly(rC)-binding protein 2
• Protein Family: Poly(rC)-binding protein
• UniProt Gene Name: PCBP2
• UniProt Synonym Gene Names: hnRNP E2
• UniProt Entry Name: PCBP2_HUMAN

NCBI Description

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP E2: Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: nucleoplasm; focal adhesion; membrane; cytoplasm; cytosol; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; enzyme binding; DNA binding; RNA binding; ubiquitin protein ligase binding

Biological Process: nuclear mRNA splicing, via spliceosome; proteasomal ubiquitin-dependent protein catabolic process; RNA splicing; innate immune response; gene expression; negative regulation of defense response to virus; defense response to virus; negative regulation of interferon type I production; mRNA metabolic process