Mouse anti-Human HLA-G Monoclonal Antibody | anti-HLA-G antibody

Anti-HLA-G Alexa Fluor488

• Gene Names: HLA-G; MHC-G
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunocytochemistry
• Purity: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: HLA-G; Monoclonal Antibody; Anti-HLA-G Alexa Fluor488; major histocompatibility complex; class I; G; anti-HLA-G antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 5A6G7
• Specificity: The mouse monoclonal antibody 5A6G7 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allows to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-HLA-G antibody: Flow Cytometry (FC/FACS) (QC tested), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: FC/FACS: Recommended dilution: 2-3ug/ml
• Conjugation: Alexa Fluor488
• Immunogen: C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References

*Poláková K, Zelezníková T, Russ G: HLA-G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb;37(2):139-45., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=polakova+,*Rebmann V, Lemaoult J, Rouas-Freiss N, Carosella ED, Grosse-Wilde H: Report of the Wet Workshop for Quantification of Soluble HLA-G in Essen, 2004. Hum Immunol. 2005 Aug;66(8):853-63., URL: https://www.ncbi.nlm.nih.gov/pubmed/16216668dopt=,*Le Rond S, Le Maoult J, Creput C, Menier C, Deschamps M, Le Friec G, Amiot L, Durrbach A, Dausset J, Carosella ED, Rouas-Freiss N: Alloreactive CD4+ and CD8+ T cells express the immunotolerant HLA-G molecule in mixed lymphocyte reactions: in vivo implications in transplanted patients. Eur J Immunol. 2004 Mar;34(3):649-60., URL: https://www.ncbi.nlm.nih.gov/pubmed/14991594dopt=,*Le Rond S, Gonzalez A, Gonzalez AS, Carosella ED, Rouas-Freiss N: Indoleamine 2,3 dioxygenase and human leucocyte antigen-G inhibit the T-cell alloproliferative response through two independent pathways. Immunology. 2005 Nov; 116(3): 297-307., URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC18024,*Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA-G Expression in Human Neural Cells after Neurotropic Viral Infections. J Virol. 2005 Dec; 79(24): 15226-15237., URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13160
*Creput C, Durrbach A, Menier C, Guettier C, Samuel D, Dausset J, Charpentier B, Carosella ED, Rouas-Freiss N. Human leukocyte antigen-G (HLA-G) expression in biliary epithelial cells is associated with allograft acceptance in liver-kidney transplantation. J Hepatol. 2003 39(4):587-94., URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgidb=p,*Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, LeDanff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 64(3):315-26., URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgidb=p

NCBI and Uniprot Product Information

• NCBI GI #: 122132
• NCBI GeneID: 3135
• UniProt Accession #: P17693
• Molecular Weight: 338
• NCBI Official Full Name: HLA class I histocompatibility antigen, alpha chain G
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, G
• NCBI Official Symbol: HLA-G
• NCBI Official Synonym Symbols: MHC-G
• NCBI Protein Information: HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; b2 microglobulin; HLA class I molecule; MHC class I antigen G; HLA-G histocompatibility antigen, class I, G
• UniProt Protein Name: HLA class I histocompatibility antigen, alpha chain G
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-G
• UniProt Synonym Gene Names: HLA-6.0; HLAG
• UniProt Entry Name: HLAG_HUMAN