Mouse anti-Human HLA-DR Monoclonal Antibody | anti-HLA-DR antibody

Mouse anti-Human HLA-DR, Purified mAb

Cat. Num. AAA8505003

• Gene Names: HLA-DQB1; IDDM1; CELIAC1; HLA-DQB
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: HLA-DR; Monoclonal Antibody; Mouse anti-Human HLA-DR; Purified mAb; anti-HLA-DR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 4ALN3
• Form/Format: Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
• Sequence Length: 42

• Applicable Applications for anti-HLA-DR antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Experimental Methods: 1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube; ; 2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature; ; 3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×); ; 4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant; ; 5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant; ; 6.Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.; 7.Repeat step 5.; 8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).; [PBS wash buffer: PBS +1% FBS +0.1% NaN3]; [Cell fixation: 2% formaldehyde solution]
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

Testing Data

Related Product Information for anti-HLA-DR antibody

HLA-DR a human class II antigen of the major histocompatibility complex(MHC), is a transmembrane glycoprotein composed of an alpha chain (36 kDa) and a beta subunit(27kDa) expressed primarily on antigen presenting cells:B cells, monocytes, macrophages and thymic epithelial cells. HLA-DR is also expressed on activated T cells. This molecule plays a major role in cellular interaction during antigen presentation.

NCBI and Uniprot Product Information

• NCBI GI #: 642238
• NCBI GeneID: 3119
• UniProt Accession #: Q29900; P01920
• Molecular Weight: 5,068 Da
• NCBI Official Full Name: HLA-DR, partial
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DQ beta 1
• NCBI Official Symbol: HLA-DQB1
• NCBI Official Synonym Symbols: IDDM1; CELIAC1; HLA-DQB
• NCBI Protein Information: HLA class II histocompatibility antigen, DQ beta 1 chain
• UniProt Protein Name: HLA-DR

NCBI Description

HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]