Mouse HLA Class 2 Antigen DR Monoclonal Antibody

HLA Class 2 Antigen DR (SLA Class II DR) (PE)

Cat. Num. AAA6251889

• Reactivity: Bovine, Porcine
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
• Purity: Purified by protein A affinity chromatography from tissue culture supernatant.
• Synonyms: HLA Class 2 Antigen DR; Monoclonal Antibody; HLA Class 2 Antigen DR (SLA Class II DR) (PE); anti-HLA Class 2 Antigen DR antibody

• Host: Mouse
• Reactivity: Bovine, Porcine
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 10D419
• Specificity: Recognizes porcine HLA Class 2 Antigen DR. Species Crossreactivity: bovine. Reacts with lymphocytes from all outbred and miniature pigs so far tested, suggesting that it recognises a monomorphic determinant of porcine HLA Class 2 Antigen DR.
• Purity/Purification: Purified by protein A affinity chromatography from tissue culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HLA Class 2 Antigen DR antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Immunogen: Porcine monocytes
• Conjugate: PE
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis on formalin fixed, paraffin embedded tissue sections using H6099-95Z for the evaluation of MHC expression. From: Wang Y, Bao J, Wu X, Wu Q, Li Y, Zhou Y, Li L, Bu H. Genipin crosslinking reduced the immunogenicity of xenogeneic decellularized porcine whole-liver matrices through regulation of immune cell proliferation and polarization. Sci Rep. 2016 Apr 21;6:24779.)

Testing Data

(Pig peripheral blood lymphocytes stained with H6099-95Z followed by Goat anti Mouse IgG:FITC)

Testing Data

(CD40L induces B cell activation. CD21+ purified B cell populations were cultured in the presence or absence of CD40L (1ug/ml), gated on live cells, and then analyzed for changes in the median fluorescence intensity (MFI) of SLA-DR (A) and CD80 (B). Cells were analyzed prior to culture and at 24hrs intervals for a period of 7 days. One representative experiment of two performed is displayed. Data shown are mean ± SEM. Statistical differences of *p )

Product Categories/Family for anti-HLA Class 2 Antigen DR antibody

Antibodies; HLA

References

1. Bullido, R. et al. (1997) Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals. Dev. Comp. Immunol. 21:311-322. 2. Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus. Clin Vaccine Immunol. 17: 503-12. 3. Ding, Q. et al. (2011) Human PD-L1-overexpressing porcine vascular endothelial cells induce functionally suppressive human CD4+CD25hiFoxp3+ Treg cells. J Leukoc Biol. 90 (1): 77-86. 4. Wang, Y. et al. (2016) Genipin crosslinking reduced the immunogenicity of xenogeneic decellularized porcine whole-liver matrices through regulation of immune cell proliferation and polarization. Sci Rep. 6: 24779. 5. Park KM et al. (2013) Generation of porcine induced pluripotent stem cells and evaluation of their major histocompatibility complex protein expression in vitro. Vet Res Commun. 37 (4): 293-301. 6. Iwase H et al. (2015) Initial in vivo experience of pig artery patch transplantation in baboons using mutant MHC (CIITA-DN) pigs. Transpl Immunol. 32 (2): 99-108. 7. Singleton, H. et al. (2016) Establishing Porcine Monocyte-Derived Macrophage and Dendritic Cell Systems for Studying the Interaction with PRRSV-1. Front Microbiol. 7: 832. 8. Zanotti, C. et al. (2015) Differential Biological Activities of Swine Interferon-alpha Subtypes. J Interferon Cytokine Res. 35 (12): 990-1002. 9. Rayat GR et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria. Xenotransplantation. 23 (1): 38-45. 10. Masek J et al. (2016) Multi-layered nanofibrous mucoadhesive films for buccal and sublingual administration of drug-delivery and vaccination nanoparticles - important step towards effective mucosal vaccines. J Control Release. Jul 25. pii: S0168-3659(16)30471-0 11. Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model. Am J Physiol Renal Physiol. 310 (4): F259-71. 12. Rahe, M.C. & Murtaugh, M.P. (2017) Interleukin-21 Drives Proliferation and Differentiation of Porcine Memory B Cells into Antibody Secreting Cells. PLoS One. 12 (1): e0171171.