Mouse anti-Human HLA Class 2 Antigen DQ7 Monoclonal Antibody | anti-HLA-C antibody

HLA Class 2 Antigen DQ7

Cat. Num. AAA603204

• Gene Names: HLA-C; HLC-C; D6S204; PSORS1; HLA-JY3
• Reactivity: Human
• Purity: Ascites; Ascites
• Synonyms: HLA Class 2 Antigen DQ7; Monoclonal Antibody; Anti -HLA Class 2 Antigen DQ7; anti-HLA-C antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: 4i125
• Specificity: Reacts to HLA Class 2 Antigen-DQ7. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a lyophilized powder in PBS, 1% BSA. Reconstitute to 100ul sterile dH2O to make a 10X stock solution.

• Application Notes: Suitable for use in Flow Cytometry, Cell Typing, Tissue Staining, and Chimerism Studies.; Dilution: Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution.
• Immunogen: HLA Class 2 Antigen-DQ7.
• Cytotoxicity: Expected Results:; Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.; Limitations:; Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.; Please contact MyBioSource for bulk requirements and licensing requests relating to production applications.

Product Categories/Family for anti-HLA-C antibody

Antibodies; Abs to HLA

NCBI and Uniprot Product Information

• NCBI GI #: 1944469
• NCBI GeneID: 3107
• UniProt Accession #: P04222; O62883; O78060; O78068; O78069; P30503; Q29743; Q29992; Q29993; Q9GIK8; Q9TQB4; P10321; P30499; P30501; P30504; P30505; P30508; P30510; Q29960; Q29963
• Molecular Weight: 40,861 Da
• NCBI Official Full Name: HLA-Cw*0304
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, C
• NCBI Official Symbol: HLA-C
• NCBI Official Synonym Symbols: HLC-C; D6S204; PSORS1; HLA-JY3
• NCBI Protein Information: HLA class I histocompatibility antigen, Cw-1 alpha chain; MHC class I antigen heavy chain HLA-C; human leukocyte antigen-C alpha chain; major histocompatibility antigen HLA-C; HLA class I histocompatibility antigen, C alpha chain
• UniProt Protein Name: HLA class I histocompatibility antigen, Cw-3 alpha chain
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-C
• UniProt Synonym Gene Names: HLAC
• UniProt Entry Name: 1C03_HUMAN

NCBI Description

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described [provided by RefSeq, Jul 2008]

Uniprot Description

HLAC iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; integral to plasma membrane; endoplasmic reticulum; early endosome membrane; plasma membrane; extracellular region; MHC class I protein complex

Molecular Function: peptide antigen binding; TAP binding; receptor binding

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; regulation of immune response; viral reproduction; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; innate immune response; immune response