Mouse anti-Human HLA Class 1 Antigen B27 Monoclonal Antibody | anti-HLA-B antibody

HLA Class 1 Antigen B27 (FITC)

Cat. Num. AAA600254

• Gene Names: HLA-B; AS; HLAB; SPDA1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Highly Purified; Purified by Ion Exchange chromatography.
• Synonyms: HLA Class 1 Antigen B27; Monoclonal Antibody; HLA Class 1 Antigen B27 (FITC); Anti -HLA Class 1 Antigen B27 (FITC); anti-HLA-B antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 3H2219
• Specificity: Recognizes HLA Class 1 Antigen B27. Affinity studies by Scatchard analysis showed that HLA-B27 antibody has a higher affinity for HLA-B27 (9.7 x 10e8M-1) than for HLA B7 (9.5 x 10e7 M-1). Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative.
• Purity/Purification: Highly Purified; Purified by Ion Exchange chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-HLA-B antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: Neat; Use 10ul to label 10e6 cells or 100ul of whole blood.; Optimal working dilutions to be determined by researcher.
• Immunogen: Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

Related Product Information for anti-HLA-B antibody

This is a cytotoxic antibody which detects HLA-B27 alloantigen. This antibody reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27+ and precipitates cell surface molecules of 43 and 12kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.

Product Categories/Family for anti-HLA-B antibody

Antibodies; Abs to HLA

NCBI and Uniprot Product Information

• NCBI GI #: 5705951
• NCBI GeneID: 3106
• UniProt Accession #: P01889; Q29638; Q29681; Q29854; Q29861; Q31613; Q5SRJ2; Q9GIX1; Q9TP95; P03989; P10319; P18463; P18464; P18465; P30460; P30461; P30462; P30464
• Molecular Weight: 40,460 Da
• NCBI Official Full Name: histocompatibility antigen alpha 1 domain
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, B
• NCBI Official Symbol: HLA-B
• NCBI Official Synonym Symbols: AS; HLAB; SPDA1
• NCBI Protein Information: major histocompatibility complex, class I, B; lymphocyte antigen; MHC class I antigen SHCHA; MHC Class I HLA heavy chain; MHC class I antigen GN00104; leukocyte antigen class I-B; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen HLA-B heavy chain; HLA class I histocompatibility antigen, B alpha chain
• UniProt Protein Name: HLA class I histocompatibility antigen, B-7 alpha chain
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-B
• UniProt Synonym Gene Names: HLAB
• UniProt Entry Name: 1B07_HUMAN

NCBI Description

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

HLAB iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; endoplasmic reticulum; integral to plasma membrane; early endosome membrane; integral to membrane; plasma membrane; MHC class I protein complex

Molecular Function: peptide antigen binding; chaperone binding; TAP binding; receptor binding

Biological Process: regulation of immune response; detection of bacterium; regulation of interleukin-12 production; viral reproduction; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; defense response; protection from natural killer cell mediated cytotoxicity; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; regulation of T cell anergy; regulation of interleukin-6 production; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; immune response