Human HIV-1 Monoclonal Antibody | anti-HIV-1 antibody

HIV-1 (gp41) Human Monoclonal Antibody (50-69)

Cat. Num. AAA588199

• Reactivity: HIV-1
• Applications: ELISA, Immunoprecipitation
• Synonyms: HIV-1; Monoclonal Antibody; HIV-1 (gp41) Human Monoclonal Antibody (50-69); anti-HIV-1 antibody

• Host: Human
• Reactivity: HIV-1
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: 50-69
• Specificity: The antibody recognizes the HIV-1 protein gp41. (amino acids 579-613)
• Form/Format: Lyophilized protein G purified in PBS pH7.4

• Applicable Applications for anti-HIV-1 antibody: ELISA (EIA), Radioimmunoprecipitation (RIP), Competition Assay
• Immunogen: The antibody was generated from HIV-infected individuals by a method based on the Epstein-Barr virus transformation of peripheral blood mononuclear cells (PBMCs) followed by fusion with heteromyeloma cells.
• Preservative: None
• Preparation and Storage: Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.; To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).; For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HIV-1 antibody

Of the two types of Human immunodeficiency virus (HIV) which have been characterized, HIV-1 is more infective and is prevalent globally. The HIV virion is encapsulated by a viral envelope that contains complexes of surface proteins, which are important for the virus to attach and fuse to infect target cells. A precursor molecule known as gp160 (glycoprotein 160) is cleaved by proteases into gp120 (glycoprotein 120) and gp41 (glycoprotein 41), glycoproteins that make up the envelope proteins of HIV. Glycoprotein gp41 is embedded in the viral membrane and can interact with other gp41 proteins. The proteins gp41 and gp120 form a complex which is thought to undergo conformational changes in order to initiate membrane fusion of the virus with a cell.

References

1. Dadachova E-Pre-clinical evaluation of a 213Bi-labeled 2556 antibody to HIV-1 gp41 glycoprotein in HIV-1 mouse models as a reagent for HIV eradication. 2. Li L-Different pattern of immunoglobulin gene usage by HIV-1 compared to non-HIV-1 antibodies derived from the same infected subject. 3. Gorny MK-Preferential use of the VH5-51 gene segment by the human immune response to code for antibodies against the V3 domain of HIV-1. 4. Nyambi PN-Conserved and exposed epitopes on intact, native, primary human immunodeficiency virus type 1 virions of group M. 5. Gorny MK-Effects of oligomerization on the epitopes of the human immunodeficiency virus type 1 envelope glycoproteins. 6. Gorny MK and Zolla-Pazner S-Recognition by human monoclonal antibodies of free and complexed peptides representing the prefusogenic and fusogenic forms of human. 7. Hioe CE-Neutralization of HIV-1 primary isolates by polyclonal and monoclonal human antibodies. 8. Laal S-Synergistic neutralization of human immunodeficiency virus type 1 by combinations of human monoclonal antibodies. 9. Zolla-Pazner S-Serotyping of primary human immunodeficiency virus type 1 isolates from diverse geographic locations by flow cytometry. 10. Xu JY-Epitope mapping of two immunodominant domains of gp41, the transmembrane protein of human immunodeficiency virus type 1. 11. Robinson WE Jr-Two immunodominant domains of gp41 bind antibodies which enhance human immunodeficiency virus type 1 infection in vitro. 12. Tyler DS-Identification of sites within gp41 that serve as targets for antibody-dependent cellular cytotoxicity by using human monoclonal antibodies. 13.-Gorny MK-Production of site-selected neutralizing human monoclonal antibodies against the third variable domain of the human immunodeficiency virus type 1. 14.-Gorny MK-Generation of human monoclonal antibodies to human immunodeficiency virus.